Anti-IGF1 抗体 (ab106836)
Key features and details
- Goat polyclonal to IGF1
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IGF1 antibody
IGF1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to IGF1 -
由来種
Goat -
アプリケーション
適用あり: WB, ICC, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig -
免疫原
Peptide with sequence
C-RSVRAQRHTD
, from the internal region of human IGF1 sequence according to NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1. -
ポジティブ・コントロール
- ICC: Human HEK-293 cells; WB: Human uterus tissue lysate; IHC-P: Human spleen tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106836の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 22 kDa).
1 hour primary incubation is recommended for this product. |
ICC |
Use a concentration of 10 µg/ml.
|
|
IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 22 kDa). 1 hour primary incubation is recommended for this product. |
ICC
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
関連疾患
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
配列類似性
Belongs to the insulin family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 610255 Dog
- Entrez Gene: 3479 Human
- Entrez Gene: 16000 Mouse
- Entrez Gene: 24482 Rat
- Omim: 147440 Human
- SwissProt: P33712 Dog
- SwissProt: P01343 Human
- SwissProt: P05019 Human
see all -
製品の状態
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA. -
別名
- IBP1 antibody
- IGF I antibody
- IGF IA antibody
see all
画像
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All lanes : Anti-IGF1 antibody (ab106836) at 0.5 µg/ml
Lane 1 : Human uterus tissue lysate in RIPA buffer
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 22 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour at room temperature. Detected by chemiluminescence.
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Immunocytochemistry analysis of paraformaldehyde fixed HEK-293 (human epithelial cell line from embryonic kidney) cells, permeabilized with 0.15% Triton. Primary incubation for 1 hour at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab106836 は 12 報の論文で使用されています。
- Wu H et al. Engineered adipose-derived stem cells with IGF-1-modified mRNA ameliorates osteoarthritis development. Stem Cell Res Ther 13:19 (2022). PubMed: 35033199
- Choi YJ et al. Ameliorative effect of Abeliophyllum distichum Nakai on benign prostatic hyperplasia in vitro and in vivo. Nutr Res Pract 16:419-434 (2022). PubMed: 35919286
- Zeng N et al. Sexual dimorphic effects of igf1 deficiency on metabolism in zebrafish. Front Endocrinol (Lausanne) 13:879962 (2022). PubMed: 35966057
- Xia H et al. Yi-Qi-Jian-Pi-Xiao-Yu-Xie-Zhuo Formula Improves Muscle Atrophy via Modulating the IGF-1/PI3K/Akt Signaling Pathway in 5/6 Nephrectomized Rats. Front Pharmacol 12:624303 (2021). PubMed: 33986663
- Ai D & Yu F LncRNA DNM3OS promotes proliferation and inhibits apoptosis through modulating IGF1 expression by sponging MiR-126 in CHON-001 cells. Diagn Pathol 14:106 (2019). PubMed: 31526393