Key features and details
- Mouse monoclonal [5D4] to IgE
- Suitable for: Sandwich ELISA
- Reacts with: Human
- Isotype: IgG2a
製品名Anti-IgE antibody [5D4]
IgE 一次抗体 製品一覧
製品の詳細Mouse monoclonal [5D4] to IgE
特異性Specific for IgE Ce2-domain specific
アプリケーション適用あり: Sandwich ELISAmore details
Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum.
特記事項Concentration varies from lot to lot and can be provided on request.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Concentration information loading...
精製度Protein A purified
特記事項（精製）Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
sELISA pair antibody
Our Abpromise guarantee covers the use of ab7295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [4F4] to IgE (HRP) (ab7294). Can be used as Capture antibody with recommended pair.|
関連性IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
- Igh2 antibody
- IGHE antibody
- IGHEP1 antibody
- Immunoglobulin heavy constant epsilon antibody
Standard curve: Capture anitbody (ab7295) at 5 ug/ml in PBS, human IgE from 100 ng/ml to 1.56 ng/ml, detection antibody (ab7294, HRP conjugated) at 0.7 ug/ml concentration.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab7295 は 1 報の論文で使用されています。
- Nguyen AT et al. DOT1L regulates dystrophin expression and is critical for cardiac function. Genes Dev 25:263-74 (2011). PubMed: 21289070