Anti-IgD 抗体 [EPR6146] (ab124795)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6146] to IgD
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IgD antibody [EPR6146]
IgD 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6146] to IgD -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide within Human IgD aa 50-150. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Human tonsil lysate, Human plasma lysates IHC-P: Human tonsil tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 4.00 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6146 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124795の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/3500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/1000 - 1/2000. |
特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
IHC-P
1/3500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/1000 - 1/2000. |
ターゲット情報
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関連性
Little is known about the normal function of IgD, and few clinical signs or symptoms are associated with its absence. Individuals with low or absent levels of IgD do not appear unusually predisposed to infections. In mice, IgD may substitute for some functions of IgM when IgM is absent. IgD is expressed on peripheral B cells and is used as a pan B cell marker. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 3495 Human
- Omim: 147170 Human
- SwissProt: P01880 Human
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別名
- Ig delta chain C region antibody
- IGHD antibody
- Immunoglobulin heavy constant delta antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling IgD with purified ab124795 at 1/3500 dilution (0.49 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-IgD antibody [EPR6146] (ab124795) at 1/1000 dilution (Purified)
Lane 1 : Human plasma lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
ab124795, at a 1/1000 dilution, staining IgD in paraffin embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-IgD antibody [EPR6146] (ab124795) at 1/1000 dilution + Human tonsil lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab124795 は 2 報の論文で使用されています。
- Moysi E et al. Altered immune cell follicular dynamics in HIV infection following influenza vaccination. J Clin Invest 128:3171-3185 (2018). PubMed: 29911996
- Qu XL et al. Establishment of a combination scoring method for diagnosis of ocular adnexal lymphoproliferative disease. PLoS One 12:e0160175 (2017). IHC-P ; Human . PubMed: 28510589