Anti-IFT46 抗体 (ab122422)
Key features and details
- Rabbit polyclonal to IFT46
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IFT46 antibody
IFT46 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IFT46 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: ETDSDSDDDD EEHGAPLEGA YDPADYEHLP VSAEIKELFQ YISRYTPQLI DLDHKLKPFI PDFIPAVGDI DAFLKVPRPD GKPDNLGLLV, corresponding to amino acids 44-133 of Human IFT46.
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ポジティブ・コントロール
- Human bronchus tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122422の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Forms part of a complex involved in intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. May play a role in chondrocyte maturation and skeletogenesis. -
配列類似性
Belongs to the IFT46 family. -
細胞内局在
Cytoplasm > cytoskeleton > cilium basal body. Cell projection > cilium. Expression is concentrated at the cilium basal body but is also detected along the length of the cilium. - Information by UniProt
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参照データベース
- Entrez Gene: 56912 Human
- SwissProt: Q9NQC8 Human
- Unigene: 533738 Human
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別名
- C11orf2 antibody
- C11orf60 antibody
- IFT46 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab122422 は 4 報の論文で使用されています。
- Shaheen R et al. Biallelic Mutations in Tetratricopeptide Repeat Domain 26 (Intraflagellar Transport 56) Cause Severe Biliary Ciliopathy in Humans. Hepatology 71:2067-2079 (2020). PubMed: 31595528
- Shaheen R et al. Bi-allelic Mutations in FAM149B1 Cause Abnormal Primary Cilium and a Range of Ciliopathy Phenotypes in Humans. Am J Hum Genet 104:731-737 (2019). PubMed: 30905400
- Anderson AE et al. Tgif1 and Tgif2 Repress Expression of the RabGAP Evi5l. Mol Cell Biol 37:N/A (2017). PubMed: 27956704
- Inaba Y et al. Transport of the outer dynein arm complex to cilia requires a cytoplasmic protein Lrrc6. Genes Cells 21:728-39 (2016). PubMed: 27353389