Anti-IDH2 (mutated R172S) 抗体 [SMab-2] - BSA and Azide free (ab264063)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [SMab-2] to IDH2 (mutated R172S) - BSA and Azide free
- Suitable for: Indirect ELISA, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free
IDH2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [SMab-2] to IDH2 (mutated R172S) - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: Indirect ELISA, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein. ICC/IF: SW1353 cells.
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特記事項
ab264063 is the carrier-free version of ab264056.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SMab-2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab264063の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Indirect ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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Indirect ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. -
関連疾患
D-2-hydroxyglutaric aciduria 2
Glioma
enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. -
配列類似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
翻訳後修飾
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 3418 Human
- Omim: 147650 Human
- SwissProt: P48735 Human
- Unigene: 596461 Human
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別名
- D2HGA2 antibody
- ICD-M antibody
- IDH antibody
see all
画像
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Western blot - Anti-IDH2 (mutated R172 S) antibody [SMab-2] (ab264056).
The loading samples are E.coil extracts containing recombinant protein respectively.
ab264056 used at a 1/1000 dilution (1.329µg/ml), followed by Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) secondary antibody at a 1/10,000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein 10ng
Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein 10ng
Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ngThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264056).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW1353 (human chondrosarcoma cell line) cells labelling IDH2 (mutated R172 S) with ab264056 at 1/50 dilution (26.58µg/ml), followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SW1353 cells.
Negative control: HeLa (PMID: 25753205).
ab179504 (YCA-R16775) Anti-beta IV Tubulin antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab264056 at a 1/50 dilution (26.58µg/ml) followed by ab150080 at a 1/1000 dilution.
Negative control 2: ab179504 at 1/200 dilution followed by ab150113 at a 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264056).
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ELISA - Anti-IDH2 (mutated R172 S) antibody [SMab-2] (ab264056).
ab264056 used at 0-5000 ng/ml, followed by an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) secondary used at 1/1000 dilution.
Antigen concentration, 100 ng/ml.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264056).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab264063 は 1 報の論文で使用されています。
- Liu X et al. A novel monoclonal antibody SMab-2 recognizes endogenous IDH2-R172S of chondrosarcoma. Biochem Biophys Res Commun 459:636-42 (2015). PubMed: 25753205