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遺伝子ノックアウト細胞株 検証済リコンビナント

Anti-IDH1 抗体 [RcMab-1] - BSA and Azide free (ab256562)

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Western blot - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
  • Western blot - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
  • Immunocytochemistry/ Immunofluorescence - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
  • Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [RcMab-1] to IDH1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-IDH1 antibody [RcMab-1] - BSA and Azide free
    IDH1 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [RcMab-1] to IDH1 - BSA and Azide free

  • 由来種

    Rat

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Recombinant fragment within Human IDH1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: O75874

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • IHC-P: Human prostatic hyperplasia tissue and kidney tissue. WB: Wild type HAP1 whole cell lysate. HeLa, PC-12, HepG2 and Raji whole cell lysate. Human and rat heart tissue lysate. ICC/IF: Wild type HAP1 cells.

  • 特記事項

    ab256562 is the carrier-free version of ab256557.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab256562の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 関連疾患

    Glioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.

  • 配列類似性

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.

  • 翻訳後修飾

    Acetylation at Lys-374 dramatically reduces catalytic activity.

  • 細胞内局在

    Cytoplasm. Peroxisome.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cytosolic NADP isocitrate dehydrogenase antibody
      • Cytosolic NADP-isocitrate dehydrogenase antibody
      • Epididymis luminal protein 216 antibody
      • Epididymis secretory protein Li 26 antibody
      • HEL-216 antibody
      • HEL-S-26 antibody
      • ICDH antibody
      • IDCD antibody
      • IDH antibody
      • IDH1 antibody
      • IDHC_HUMAN antibody
      • IDP antibody
      • IDPC antibody
      • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic antibody
      • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
      • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
      • NADP dependent isocitrate dehydrogenase cytosolic antibody
      • NADP dependent isocitrate dehydrogenase peroxisomal antibody
      • NADP(+)-specific ICDH antibody
      • Oxalosuccinate decarboxylase antibody
      • PICD antibody
      see all

    画像

    • Western blot - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
      Western blot - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
      All lanes : Anti-IDH1 antibody [RcMab-1] (ab256557) at 1.479 µg/ml

      Lane 1 : Wild type HAP1 whole cell lysate
      Lane 2 : IDH1 knockout HAP1 whole cell lysate
      Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 5 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

      Predicted band size: 46 kDa


      Exposure time: 8 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

    • Western blot - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
      Western blot - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
      All lanes : Anti-IDH1 antibody [RcMab-1] (ab256557) at 1.479 µg/ml

      Lane 1 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
      Lane 2 : Rat heart tissue lysate
      Lane 3 : Human heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

      Predicted band size: 46 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 3 minutes; Lanes 2-3: 3 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

      Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labeling IDH1 with ab256557 at 1/500 dilution (14.79µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on human prostatic hyperplasia. The section was incubated with ab256557 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling IDH1 with ab256557 at 1/500 dilution (14.79µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on human kidney tissue. The section was incubated with ab256557 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

      Immunohistochemical analysis of paraffin-embedded human normal cerebrum tissue labeling IDH1 with ab256557 at 1/500 dilution (14.79µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). The section was incubated with ab256557 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Negative control: No staining on human normal cerebrum.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

    • Immunocytochemistry/ Immunofluorescence - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
      Immunocytochemistry/ Immunofluorescence - Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized wild-type HAP1 cells labelling IDH1 with ab256557 at 1/20 dilution (73.95µg/ml), followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

      Confocal image showing positive staining in wild-type HAP1 cells, while no staining in IDH1 knockout HAP1 cells.

      ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only controls.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256557).

    • Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)
      Anti-IDH1 antibody [RcMab-1] - BSA and Azide free (ab256562)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab256562 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab256562 は 1 報の論文で使用されています。

    • Kato Kaneko M  et al. Establishment of a multi-specific monoclonal antibody MsMab-1 recognizing both IDH1 and IDH2 mutations. Tohoku J Exp Med 230:103-9 (2013). PubMed: 23782684

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