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Anti-IDH1 抗体 [HMab-2] - BSA and Azide free (ab256563)

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Western blot - Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)
  • ELISA - Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)
  • Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [HMab-2] to IDH1 - BSA and Azide free
  • Suitable for: WB, ELISA
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-IDH1 antibody [HMab-2] - BSA and Azide free
    IDH1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [HMab-2] to IDH1 - BSA and Azide free

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, ELISAmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein.

  • 特記事項

    ab256563 is the carrier-free version of ab256558.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab256563の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
ELISA
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
ELISA
Use at an assay dependent concentration.

ターゲット情報

  • 関連疾患

    Glioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.

  • 配列類似性

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.

  • 翻訳後修飾

    Acetylation at Lys-374 dramatically reduces catalytic activity.

  • 細胞内局在

    Cytoplasm. Peroxisome.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cytosolic NADP isocitrate dehydrogenase antibody
      • Cytosolic NADP-isocitrate dehydrogenase antibody
      • Epididymis luminal protein 216 antibody
      • Epididymis secretory protein Li 26 antibody
      • HEL-216 antibody
      • HEL-S-26 antibody
      • ICDH antibody
      • IDCD antibody
      • IDH antibody
      • IDH1 antibody
      • IDHC_HUMAN antibody
      • IDP antibody
      • IDPC antibody
      • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic antibody
      • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
      • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
      • NADP dependent isocitrate dehydrogenase cytosolic antibody
      • NADP dependent isocitrate dehydrogenase peroxisomal antibody
      • NADP(+)-specific ICDH antibody
      • Oxalosuccinate decarboxylase antibody
      • PICD antibody
      see all

    画像

    • Western blot - Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)
      Western blot - Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)

      ab256558 used at 1.263µg/ml.
      Goat Anti-Rat IgG (H+L), HRP) (ab205720) used as the secondary at a 1/10,000 dilution.
      Blocking/Dilution buffer:5% NFDM/TBST.

      Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein 10ng
      Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
      Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
      Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
      Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
      Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
      Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
      Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein 10ng
      Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
      Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
      Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
      Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
      Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
      Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng

      The loading samples are E.coil extracts containing recombinant protein respectively.
      Exposure time of 3 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256558).

    • ELISA - Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)
      ELISA - Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)

      ELISA - Anti-IDH1 antibody [HMab-2].

      ab256558 used at 0-5000 ng/ml.

      Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) was used as the secondary at a 1/1000 dilution.

      Antigen concentration 1000 ng/ml.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256558).

    • Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)
      Anti-IDH1 antibody [HMab-2] - BSA and Azide free (ab256563)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab256563 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab256563 は 1 報の論文で使用されています。

    • Fujii Y  et al. A high-sensitive HMab-2 specifically detects IDH1-R132H, the most common IDH mutation in gliomas. Biochem Biophys Res Commun 466:733-9 (2015). PubMed: 26381180

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