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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-IDH1 抗体 [EPR12296] - BSA and Azide free (ab214803)

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Western blot - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
  • Western blot - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
  • Western blot - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
  • Immunoprecipitation - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
  • Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12296] to IDH1 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-IDH1 antibody [EPR12296] - BSA and Azide free
    IDH1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12296] to IDH1 - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • アプリケーション

    適用あり: IHC-P, WB, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Cow, Orangutan

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HAP1, HepG2, and HeLa cell lysates. IHC-P: Human gastric cancer tissue. IP: HepG2 whole cell lysate. WB: Raji, HepG2 and Neuro-2a whole cell lysates. Rat kidney lysate.

  • 特記事項

    ab214803 is the carrier-free version of ab172964.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab214803の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
IP
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 関連疾患

    Glioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.

  • 配列類似性

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.

  • 翻訳後修飾

    Acetylation at Lys-374 dramatically reduces catalytic activity.

  • 細胞内局在

    Cytoplasm. Peroxisome.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Cytosolic NADP isocitrate dehydrogenase antibody
    • Cytosolic NADP-isocitrate dehydrogenase antibody
    • Epididymis luminal protein 216 antibody
    • Epididymis secretory protein Li 26 antibody
    • HEL-216 antibody
    • HEL-S-26 antibody
    • ICDH antibody
    • IDCD antibody
    • IDH antibody
    • IDH1 antibody
    • IDHC_HUMAN antibody
    • IDP antibody
    • IDPC antibody
    • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic antibody
    • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
    • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
    • NADP dependent isocitrate dehydrogenase cytosolic antibody
    • NADP dependent isocitrate dehydrogenase peroxisomal antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    • PICD antibody
    see all

画像

  • Western blot - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
    Western blot - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
    Anti-IDH1 antibody [EPR12296] (ab172964) at 1/1000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa

  • Western blot - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
    Western blot - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
    All lanes : Anti-IDH1 antibody [EPR12296] (ab172964) at 1/1000 dilution (Purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 47 kDa

  • Western blot - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
    Western blot - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)

    This WB data was generated using the same anti-IDH1 antibody clone, EPR12296, in a different buffer formulation (cat# ab172964).

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: IDH1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab172964 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab172964 was shown to specifically react with IDH1 in wild-type HAP1 cells. No band was observed when IDH1 knockout samples were used. Wild-type and IDH1 knockout samples were subjected to SDS-PAGE.  Ab172964 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)

    This data was developed using ab172964, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric cancer tissue sections labeling IDH1 with purified ab172964 at 1/200 dilution (0.60 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunoprecipitation - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
    Immunoprecipitation - Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)

    This data was developed using ab172964, the same antibody clone in a different buffer formulation.
    Purified ab172964 at 1/20 dilution (0.6µg) immunoprecipitating IDH1 in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate.
    Lane 2 (+): ab172964 + HepG2 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172964 in HepG2 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.

  • Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)
    Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)

参考文献 (0)

ab214803 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab214803 は論文での使用が確認できていません。

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