リコンビナントRabMAb

Anti-ICOS Ligand/ICOSL 抗体 [EPR23289-152] - BSA and Azide free (ab272697)

Submit a review Submit a question
Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)
  • Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)
  • Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23289-152] to ICOS Ligand/ICOSL - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free
    ICOS Ligand/ICOSL 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23289-152] to ICOS Ligand/ICOSL - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cytmore details
    適用なし: ICC/IF,IHC-P,IP or WB

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Flow Cyt: Jurkat, HUVEC cells.

  • 特記事項

    ab272697 is the carrier-free version of ab233554. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23289-152

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab272697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for ICC/IF,IHC-P,IP or WB.

    • ターゲット情報

    • 機能

      Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.

    • 組織特異性

      Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.

    • 配列類似性

      Belongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • B7 H2 antibody
        • B7 homolog 2 antibody
        • B7 homologue 2 antibody
        • B7 like protein Gl50 antibody
        • B7 related protein 1 antibody
        • B7-H2 antibody
        • B7-like protein Gl50 antibody
        • B7-related protein 1 antibody
        • B7H2 antibody
        • B7RP 1 antibody
        • B7RP-1 antibody
        • B7RP1 antibody
        • CD 275 antibody
        • CD275 antibody
        • CD275 antigen antibody
        • GL 50 antibody
        • GL50 antibody
        • ICOS L antibody
        • ICOS LG antibody
        • ICOS ligand antibody
        • ICOSL antibody
        • ICOSL_HUMAN antibody
        • Icoslg antibody
        • Inducible T cell co stimulator ligand antibody
        • KIAA0653 antibody
        • LICOS antibody
        • Transmembrane protein B7 H2 ICOS ligand antibody
        see all

      画像

      • Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)
        Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)

        Flow cytometric analysis of HUVEC (Human umbilical vein endothelial cell) cells treated with 200U/ml TNF alpha for 24h (Red) / Untreated control (Green) cells labelling ICOS Ligand/ICOSL with ab233554 at 1/50 dilution (1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells (PMID: 16210590).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233554).

      • Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)
        Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)

        Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / Raji (Human Burkitt's lymphoma B lymphocyte, Right) cells labeling ICOS Ligand/ICOSL with ab233554 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat (PMID: 21851236). Gated on viable cells.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233554).

      • Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)
        Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab272697 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab272697 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      There are currently no Customer reviews or Questions for ab272697.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com