Anti-ICOS 抗体 [SP98] - BSA and Azide free (ab236226)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP98] to ICOS - BSA and Azide free
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ICOS antibody [SP98] - BSA and Azide free
ICOS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP98] to ICOS - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IHC-Frmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human ICOS (C terminal). The exact sequence is proprietary.
Database link: Q9Y6W8 -
ポジティブ・コントロール
- IHC-P: Human lymph node, pancreatic adenocarcinoma, thymus and tonsil tissue. IHC-Fr: Frozen human tonsil tissue sections
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特記事項
ab236226 is the carrier-free version of ab105227.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP98 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-ICOS antibody [SP98] (ab105227)
- Alexa Fluor® 488 Anti-ICOS antibody [SP98] (ab311010)
- Alexa Fluor® 647 Anti-ICOS antibody [SP98] (ab311138)
- Alexa Fluor® 594 Anti-ICOS antibody [SP98] (ab311775)
- Alexa Fluor® 568 Anti-ICOS antibody [SP98] (ab313056)
- Alexa Fluor® 555 Anti-ICOS antibody [SP98] (ab313256)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236226の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at RT for 20 min. |
|
IHC-Fr |
Use at an assay dependent concentration.
|
特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at RT for 20 min. |
IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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機能
Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. -
組織特異性
Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus. -
関連疾患
Immunodeficiency, common variable, 1 -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 29851 Human
- Entrez Gene: 54167 Mouse
- Omim: 604558 Human
- SwissProt: Q9Y6W8 Human
- SwissProt: Q9WVS0 Mouse
- Unigene: 56247 Human
- Unigene: 42044 Mouse
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別名
- Activation inducible lymphocyte immunomediatory molecule antibody
- Activation-inducible lymphocyte immunomediatory molecule antibody
- AILIM antibody
see all
画像
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This image was generated using ab105227, the same clone, but with a different buffer formulation.
IHC image of ICOS staining in a section of frozen normal human tonsil* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab105227, 5ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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This image was generated using ab105227, the same clone, but with a different buffer formulation.
Negative control image: IHC image of ICOS staining in a section of frozen normal human placenta* performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab105227, 5ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling ICOS with ab105227 at 1/100 dilution (0.28 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically positive staining in the germinal center of human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab105227 for 30 mins at room temperature.This image was generated using ab105227, the same clone, but with a different buffer formulation.
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Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab105227).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab236226 は論文での使用が確認できていません。