Key features and details
- Mouse monoclonal [ICAM3.1] to ICAM3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品名Anti-ICAM3 antibody [ICAM3.1]
ICAM3 一次抗体 製品一覧
製品の詳細Mouse monoclonal [ICAM3.1] to ICAM3
アプリケーション適用あり: IHC-Pmore details
ICAM 3Fc chimeric fusion protein.
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration information loading...
精製度Protein A purified
The Abpromise guarantee
Flow Cyt: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 66 kDa.
ICC/IF: Use at an assay dependent dilution From (PubMed:17353362)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
配列類似性Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
翻訳後修飾Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.
- Information by UniProt
- CD50 antibody
- CDW 50 antibody
- CDw50 antibody
Ab10804 staining Human malignant stomach. Staining is localised to the membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab10804 は 4 報の論文で使用されています。
- Blight BJ et al. Cell Adhesion Molecules are Upregulated and May Drive Inflammation in Chronic Rhinosinusitis with Nasal Polyposis. J Asthma Allergy 14:585-593 (2021). PubMed: 34079296
- Mintz PJ et al. Exploiting human CD34+ stem cell-conditioned medium for tissue repair. Mol Ther 22:149-59 (2014). PubMed: 23985698
- Heasman SJ et al. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol 190:553-63 (2010). ICC/IF ; Human . PubMed: 20733052
- Gérard A et al. The Par polarity complex regulates Rap1- and chemokine-induced T cell polarization. J Cell Biol 176:863-75 (2007). PubMed: 17353362