Anti-ICAM1 抗体 [MEM-111] (ab2213)
Key features and details
- Mouse monoclonal [MEM-111] to ICAM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-ICAM1 antibody [MEM-111]
ICAM1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-111] to ICAM1 -
由来種
Mouse -
特異性
his antibody reacts with human CD54 antigen.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Tissue/ cell preparation (Human). (Burkitt's lymphoma cell line RAJI).
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ポジティブ・コントロール
- For WB it is highly recommended to use TNF-alpha activated HUVEC cells as a positive control.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
MEM-111 -
ミエローマ
unknown -
アイソタイプ
IgG2a -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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sELISA pair antibody
アプリケーション
Our Abpromise guarantee covers the use of ab2213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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WB | Use at an assay dependent concentration. Use under non-reducing conditions. |
ターゲット情報
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機能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
配列類似性
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
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別名
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
画像
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All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213)
Lane 1 : TNF-alpha activated HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : TNF-alpha nonactivated HUVEC cells
Performed under non-reducing conditions.
Observed band size: 90 kDa why is the actual band size different from the predicted?Lower bands represent tubulin as a loading control.
プロトコール
参考文献 (42)
ab2213 は 42 報の論文で使用されています。
- Lima Moura S et al. Matrix Effect in the Isolation of Breast Cancer-Derived Nanovesicles by Immunomagnetic Separation and Electrochemical Immunosensing-A Comparative Study. Sensors (Basel) 20:N/A (2020). PubMed: 32054015
- Nam JE et al. Baicalin attenuates fibrogenic process in human renal proximal tubular cells (HK-2) exposed to diabetic milieu. Life Sci 254:117742 (2020). PubMed: 32360619
- Engleitner S et al. Feed-back loops integrating RELA, SOX18 and FAK mediate the break-down of the lymph-endothelial barrier that is triggered by 12(S)-HETE. Int J Oncol 56:1034-1044 (2020). PubMed: 32319559
- Sudaryatma PE et al. Bovine Respiratory Syncytial Virus Decreased Pasteurella multocida Adherence by Downregulating the Expression of Intercellular Adhesion Molecule-1 on the Surface of Upper Respiratory Epithelial Cells. Vet Microbiol 246:108748 (2020). PubMed: 32605748
- Potor L et al. The Fungal Iron Chelator Desferricoprogen Inhibits Atherosclerotic Plaque Formation. Int J Mol Sci 21:N/A (2020). PubMed: 32635347