Anti-ICAM1 抗体 [MEM-111] (ab2213)

Reviews (12)Q&A (23)References (51)
Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213)

    Key features and details

    • Mouse monoclonal [MEM-111] to ICAM1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG2a

    製品の概要

    • 製品名

      Anti-ICAM1 antibody [MEM-111]
      ICAM1 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [MEM-111] to ICAM1

    • 由来種

      Mouse

    • 特異性

      his antibody reacts with human CD54 antigen.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human ICAM1. Burkitt's lymphoma cell line RAJI

    • ポジティブ･コントロール

      • For WB it is highly recommended to use TNF-alpha activated HUVEC cells as a positive control.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab2213の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (3)
    Use at an assay dependent concentration. Use under non-reducing conditions.
    特記事項
    WB
    Use at an assay dependent concentration. Use under non-reducing conditions.

    ターゲット情報

    • 機能

      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

    • 配列類似性

      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

    • 翻訳後修飾

      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Antigen identified by monoclonal antibody BB2 antibody
        • BB 2 antibody
        • BB2 antibody
        • CD 54 antibody
        • CD_antigen=CD54 antibody
        • CD54 antibody
        • Cell surface glycoprotein P3.58 antibody
        • Human rhinovirus receptor antibody
        • ICAM 1 antibody
        • ICAM-1 antibody
        • ICAM1 antibody
        • ICAM1_HUMAN antibody
        • intercellular adhesion molecule 1 (CD54) antibody
        • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
        • Intercellular adhesion molecule 1 antibody
        • Major group rhinovirus receptor antibody
        • MALA 2 antibody
        • MALA2 antibody
        • MyD 10 antibody
        • MyD10 antibody
        • P3.58 antibody
        • Surface antigen of activated B cells antibody
        • Surface antigen of activated B cells, BB2 antibody
        see all

      画像

      • Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213)
        Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213)
        All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213)

        Lane 1 : TNF-alpha activated HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
        Lane 2 : TNF-alpha nonactivated HUVEC cells

        Performed under non-reducing conditions.

        Observed band size: 90 kDa why is the actual band size different from the predicted?



        Lower bands represent tubulin as a loading control.

      参考文献 (51)

      ab2213 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2213 は 51 報の論文で使用されています。

      • Zhang G  et al. OxLDL/ß2GPI/anti-ß2GPI Ab complex induces inflammatory activation via the TLR4/NF-?B pathway in HUVECs. Mol Med Rep 23:N/A (2021). PubMed: 33355374
      • Bauza G  et al. Improving the immunosuppressive potential of articular chondroprogenitors in a three-dimensional culture setting. Sci Rep 10:16610 (2020). PubMed: 33024130
      • Jian D  et al. METTL14 aggravates endothelial inflammation and atherosclerosis by increasing FOXO1 N6-methyladeosine modifications. Theranostics 10:8939-8956 (2020). PubMed: 32802173
      • Li X  et al. The protective effect and mechanism of lentinan on acute kidney injury in septic rats. Ann Transl Med 8:883 (2020). PubMed: 32793727
      • Lima Moura S  et al. Matrix Effect in the Isolation of Breast Cancer-Derived Nanovesicles by Immunomagnetic Separation and Electrochemical Immunosensing-A Comparative Study. Sensors (Basel) 20:N/A (2020). PubMed: 32054015
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 35 Abreviews or Q&A

      Blocking abreview for Anti-ICAM1 antibody [MEM-111]

       Excellent
      Abreviews
      Application
      Blocking
      Sample
      Streptococcus pneumoniae Cell (Primary Bronchial Epithelial cells)
      Specification
      Primary Bronchial Epithelial cells
      Read More

      DR. Selvarani Vimalanathan

      Verified customer

      投稿 Aug 03 2018

      Immunocytochemistry abreview for Anti-ICAM1 antibody [MEM-111]

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cultured Cells (BEAS-2B Epithelial cells)
      Permeabilization
      Yes - 0.5% saponin
      Specification
      BEAS-2B Epithelial cells
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      DR. Selvarani Vimalanathan

      Verified customer

      投稿 Aug 04 2017

      Western blot abreview for Anti-ICAM1 antibody [MEM-111]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Cancer cell lines total lysate)
      Gel Running Conditions
      Reduced Denaturing (10%)
      Loading amount
      20 µg
      Specification
      Cancer cell lines total lysate
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Dec 07 2015

      Western blot abreview for Anti-ICAM1 antibody [MEM-111]

       Inconclusive
      Abreviews
      Application
      Western blot
      Loading amount
      40 µg
      Gel Running Conditions
      Non-reduced Non-Denaturing (Native)
      Sample
      Rhesus monkey Cell lysate - whole cell (RF/6A cells from ATCC)
      Specification
      RF/6A cells from ATCC
      Treatment
      5.5 mM glucose, 30 mM glucose, 30 mM glucose + 0.1 mM BAPN for 10 days
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 May 26 2014

      Immunohistochemistry (Frozen sections) abreview for Anti-ICAM1 antibody [MEM-111]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rat Tissue sections (rat cortex (injured))
      Specification
      rat cortex (injured)
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.1% triton X-100 in blocking buffer
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Mar 20 2013

      Question

      I hope this email finds you well. I was just writing to you with regards to an order I recently placed. This was for an anti-ICAM1 antibody [MEM-111] (catalogue number ab2213). On the datasheet provided and on the vial it informs me that there should be 100ug at 1mg/ml, however when aliquoting the antibody I only had 90ul instead of 100ul, so had 10ug less. I thought you should know this to avoid any further issues in the future when sending out antibodies. I have been buying all my antibodies from you and this has been an isolated issue so perhaps it is a product specific problem with this item, but I thought I should raise the issue with you.
      Kind regards,

      Read More
      Answer

      I sorry to hear about the volume of the antibody; we always fill vials 4-5ul extra so quick spin is required to extract the whole volume. I am sure you have given a quick spin to vial before opening it, if it hasn't been done then it will worth to try.

      On the other hand I have let our logistic team know about this issue and I can reassure you that they have taken the necessary actions.

      Read More

      Question

      We used ICAM-1 (Ab, ab2213), but this antibody gives many unspecific bands. I tried it in many different conditions, however, it still gives me the same results.
      Please find attached

      Thanks,

      Read More
      Answer

      Thank you for your enquiry.

      As the Swiss-Prot database highlights, the mature ICAM1 protein is heavily N-Glycosylated. There at least 9 potential site for this type of post-translational modification which can significantly alter the band size. I would advise you to consult these sites for further information:

      http://www.uniprot.org/uniprot/P05362

      http://www.uniprot.org/uniprot/Q00238

      If you need any further assistance in the future, please do not hesitate to contact me.

      Read More

      Question

      Thank you very much,

      We used ICAM-1 (Ab, ab2213), but this antibody gives many unspecific bands. I tried it in many different conditions, however, it still gives me the same results.

      · Lysis buffer - would be very important to know

      We are using special a kit from abcam ab65400 (Plasma membrane protein extraction kit) in which lysis buffer is included.

      · Amount of protein loaded - (protein endogenously expressed or transfected cells)

      20-30 μg protein per lane.

      · Electrophoresis and transfer conditions

      The samples were run at 100 and 150 V, one dimensional SDS-PAGE gel was used. For transfering, Protein transfer verifiedusing Ponceau red.

      · Blocking conditions

      We used 5% and 3% fat free dry milk and also we tried 5% and 3% BSA. Blocking time, once 1 hour and in other run was 30 minutes at 25˚C

      · Primary antibody conditions (diluent/dilution, incubation time/temperature)

      Concentration or dilution

      1/1000 to 1/250 under reducing and non-reducing condition.

      · Diluent buffer

      1X PBS+0.1% Tween in 5% BSA.

      · Incubation time

      Once over night at 4C and another 1 hour and 30 minutes at room temperature.

      · Secondary antibody conditions (diluent/dilution, incubation time/temperature)

      Secondary antibody species gout which reacts against mouse antibodies, the dilution is from 1/10000 to 1/20000 in 1X PBS+0.1% Tween in 5% BSA for one hour at 25˚C.

      Wash steps and washing buffer: 1X PBS+0.1% Tween 3 times each one 10 minutes.

      · Detection method:

      ECL (super signal west pico chemiluminescent)

      · Positive control
      I used Calnexin, and Na/K ATPase (ab7671) as membrane markerThank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

      Read More
      Answer

      Thank you for your response.

      Could you please specify the size of the non-specific bands? Are they lower or higher than the expected size (58 kDa)? Could you attach an image (saved as a jpeg file) to your e-mail so that I can have a look at the bands.

      I look forward to hearing from you soon.

      Read More

      Question

      Dear,
      I ordered the following antibodies and received them at the end of September:
      - Ab54426 (anti-CD62P)
      - Ab91353 (Mouse IgG1 – isotype control)
      - Ab 18981 (anti-CD62E)
      - Ab27478 (rabbit polyclonal IgG)
      - Ab2213 (anti-ICAM1)

      I stored them at +4oC till today, but I have noticed today that your storage instructions recommend to aliquot and store most of the antibodies at -20oC or -80oC.
      Could this have had an effect on my stainings?
      Kind regards,

      Read More
      Answer

      Thank you for your enquiry.

      I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

      The others I would recommend to aliquot and freeze immediately. Keeping them at 4oC for a few weeks should not significantly affect the staining. However, we would recommend to follow the individual storage instructions provided on the individual datasheets as soon as possible after delivery. This will help to ensure that the antibody remains stable and that we can continue to provide our guarantee.

      I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

      Read More

      Question

      Phone call reporting difficulty in using the Anti-ICAM1 antibody [MEM-111] (ab2213) in ICC.

      Read More
      Answer

      Thank you for calling us yesterday and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

      As we discussed on the phone, I have attached our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results.

      This information will also allow us to investigate this case internally and initiate additional testing where necessary.

      I look forward to receiving your reply.

      Read More

      1-10 of 35 Abreviews or Q&A

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