遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-ICAM1 抗体 [EP1442Y] (ab53013)

Submit a review Q&A (5)References (37)
Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] (ab53013)
  • Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
  • Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
  • Anti-ICAM1 antibody [EP1442Y] (ab53013)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1442Y] to ICAM1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-ICAM1 antibody [EP1442Y]
    ICAM1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1442Y] to ICAM1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human ICAM1 aa 1-100 (N terminal). The exact sequence is proprietary.
    (Peptide available as ab218845)

  • ポジティブ･コントロール

    • WB: HeLa, Ramos and Raji whole cell lysate; Raji cell membrane lysate. IHC-P: Human tonsil tissue.

  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 89 kDa (predicted molecular weight: 58 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • ターゲット情報

    • 機能

      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

    • 配列類似性

      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

    • 翻訳後修飾

      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Antigen identified by monoclonal antibody BB2 antibody
        • BB 2 antibody
        • BB2 antibody
        • CD 54 antibody
        • CD_antigen=CD54 antibody
        • CD54 antibody
        • Cell surface glycoprotein P3.58 antibody
        • Human rhinovirus receptor antibody
        • ICAM 1 antibody
        • ICAM-1 antibody
        • ICAM1 antibody
        • ICAM1_HUMAN antibody
        • intercellular adhesion molecule 1 (CD54) antibody
        • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
        • Intercellular adhesion molecule 1 antibody
        • Major group rhinovirus receptor antibody
        • MALA 2 antibody
        • MALA2 antibody
        • MyD 10 antibody
        • MyD10 antibody
        • P3.58 antibody
        • Surface antigen of activated B cells antibody
        • Surface antigen of activated B cells, BB2 antibody
        see all

      画像

      • Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
        Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
        All lanes : Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/1000 dilution

        Lane 1 : Wild-type HeLa cell lysate
        Lane 2 : ICAM1 knockout HeLa cell lysate
        Lane 3 : Raji cell lysate
        Lane 4 : Ramos cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

        Predicted band size: 58 kDa
        Observed band size: 90 kDa
        why is the actual band size different from the predicted?



        Lanes 1-4: Merged signal (red and green). Green - ab53013 observed at 90 kDa. Red - loading control ab8245 observed at 36 kDa.

         ab53013 Anti-ICAM1 antibody [EP1442Y]  was shown to specifically react with ICAM1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261742 (knockout cell lysate ab256947) was used. Wild-type and ICAM1 knockout samples were subjected to SDS-PAGE. ab53013 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] (ab53013)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] (ab53013)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling ICAM1 with ab53013 at 1/1000. Heat mediated antigen retrieval was performed using ab93684, Tris/EDTA buffer pH 9. A ready to use HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.

      • Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
        Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
        All lanes : Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/1000 dilution

        Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
        Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
        Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
        Lane 4 : THP1 (Human acute monocytic leukemia cell line) + LPS Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 58 kDa
        Observed band size: 100 kDa why is the actual band size different from the predicted?


        Exposure time: 20 minutes


        s produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab53013 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

      • Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
        Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
        Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/2000 dilution + Raji cell lysate at 10 µg

        Secondary
        HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 58 kDa
        Observed band size: 89 kDa why is the actual band size different from the predicted?

      • Anti-ICAM1 antibody [EP1442Y] (ab53013)
        Anti-ICAM1 antibody [EP1442Y] (ab53013)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (37)

      ab53013 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab53013 は 37 報の論文で使用されています。

      • Chen S  et al. ICAM1 Regulates the Development of Gastric Cancer and May Be a Potential Biomarker for the Early Diagnosis and Prognosis of Gastric Cancer. Cancer Manag Res 12:1523-1534 (2020). PubMed: 32184657
      • Wu W  et al. Lnc-SNHG16/miR-128 axis modulates malignant phenotype through WNT/ß-catenin pathway in cervical cancer cells. J Cancer 11:2201-2212 (2020). PubMed: 32127947
      • Shi X  et al. LINC01093 Upregulation Protects against Alcoholic Hepatitis through Inhibition of NF-?B Signaling Pathway. Mol Ther Nucleic Acids 17:791-803 (2019). PubMed: 31450097
      • Liu Y & Tie L Apolipoprotein M and sphingosine-1-phosphate complex alleviates TNF-a-induced endothelial cell injury and inflammation through PI3K/AKT signaling pathway. BMC Cardiovasc Disord 19:279 (2019). PubMed: 31791242
      • Zhang W  et al. Effect of PA-MSAH preprocessing on the expression of TLR-4-NF-?B pathway and inflammatory factors in the intestinal tract of rats with septic shock. Exp Ther Med 17:2567-2574 (2019). PubMed: 30906447
      View all Publications for this product

      レビューと Q&A

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      レビューを送る 質問を送る

      1-5 of 5 Abreviews or Q&A

      Question

      Product code: 52895
      Lot number: GR27346-6
      Inquiry: I have noticed that you provide a concentration for some of your antibodies whilst others simply have a volume? I am using the following antibodies.. CD11a - ab52895, Lot GR27346-6 CD54 - ab53013 Lot GR10519-3 Which just have a volume, Do you have access to concentration information for these antibodies? Also is this likely to change if I receive vials from a different 'lot' in the future? Thanks,

      Read More
      Answer

      Thank you for contacting us.

      The concentration for lot GR27346-6 is XXXX mg/ml.

      The conc. for lot GR10519-3 is XXXX mg/ml.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      No signal in IHC on human tissue

      Read More
      Answer

      Thank you for calling Abcam earlier today.

      I am sorry that you were having issue with ab53013 in IHC. As we discussed on the telephone, I am sending you a vial of ab109361 as a free of charge replacement. Your new order number for tracking purposes only is *.

      If there is anything else I can help you with, or if you experience any issues with the replacement antibody, then please let me know.

      Read More

      Question

      Thank you for your below email and for the offer of a free replacement. Our customer has accepted your offer.

      Please include the free replacement in one of our next shipments.

      Thank you in advance.

      Kind regards,

      Read More
      Answer

      Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

      Weare pleased to arrange a replacement product free of charge. I have added now one free unit of ab53013,xx We are currently awaiting the vial to be transferred from the US office.To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should youthe customer stillbe experiencing difficulties, or if you have any further questions, please do not hesitate to let us know

      Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

      Read More

      Question

      Dear sir/madam,

      In accordance with your request, we have filled in the technical questionnaire. We hope this will give you all the information needed to understand the problem.

      We look forward to hearing your response.

      Best regards,

      Read More
      Answer

      Thank you very muchfor taking time to complete our questionnaire and the additional information. Even though we have not yet tested this antibody in frozen sections, I would expect the new batch to work as the previous one as this is a monoclonal antibody. Indeed it appears that you may have received a faulty vial. I am very sorry for this inconvenience. As said before, we do not have received any negative feedback on this antibody during the last three years. The vial you received might has been damaged during the transport or storage.

      I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation. We do have currently the lot GR10519-7 in stock in our US office. Would you like to receive a new vial of this lot?

      Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

      Read More

      Question

      One of our customers is using the anti ICAM-1 antibody Ab53013. Previously they had a good staining on both IHC-P/F of human skin biopsies with Lot.# GR10519-2

      With the use of the new lot.# GR10519-6 they did not get any staining, while the secondary antibody worked well on other antibodies in the same setting.

      Please advise on how to handle this problem. Is there any problem described for this lot.#?

      Thank you in advance.



      Best regards,

      Read More
      Answer

      Thank you for contacting us.

      I am sorry to hear you are experiencing difficulties with one of our products. We have not received any product complaints of this antibody during the past three years and have also no other indication that there might a problem with a batch. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.


      Even though in this case it seems to be a straigthforward batch issue, I would nevertheless appreciate if we could gather more details of the experimental procedure. It should not take more than 5 to 10 minutes to the customer. I am attaching therefore our questionnaire which will help to put together somefurther information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results - which I do not expect in this case. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


      I look forward to receiving your reply.

      Read More

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      For licensing inquiries, please contact partnerships@abcam.com