Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
40 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-1% tween 20-5% milk
Secondary antibody
Secondary antibody
None used
Detection
Detection method
avidine-peroxidase
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 100 kDa
Additional data
Additional Notes
This antibody recognizes mouse ICAM-1 from brain tissues in the conditions previously described. First lane is the sample and second lane is the molecular weight marker.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alicia Moreno
Verified customer
投稿 Sep 06 2010