Anti-ICAM1 抗体 [15.2] (ab20)

Reviews (1)Q&A (2)References (25)
Western blot - Anti-ICAM1 antibody [15.2] (ab20)

    Key features and details

    • Mouse monoclonal [15.2] to ICAM1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-ICAM1 antibody [EP1442Y] (ab53013)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-ICAM1 antibody [15.2]
      ICAM1 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [15.2] to ICAM1

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WBmore details
      適用なし: IHC-P

    • 種交差性

      交差種: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human ICAM1. Human monocytes.

    • ポジティブ･コントロール

      • WB: SK N BE, Saos-2 and HT1080 whole cell lysate. Flow Cytometry: HK-2 cells stimulated with TGF-beta1. IHC: Human amniotic membrane tissues.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab20の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
    特記事項
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
    追加情報
    Is unsuitable for IHC-P.

    ターゲット情報

    • 機能

      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

    • 配列類似性

      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

    • 翻訳後修飾

      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Antigen identified by monoclonal antibody BB2 antibody
        • BB 2 antibody
        • BB2 antibody
        • CD 54 antibody
        • CD_antigen=CD54 antibody
        • CD54 antibody
        • Cell surface glycoprotein P3.58 antibody
        • Human rhinovirus receptor antibody
        • ICAM 1 antibody
        • ICAM-1 antibody
        • ICAM1 antibody
        • ICAM1_HUMAN antibody
        • intercellular adhesion molecule 1 (CD54) antibody
        • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
        • Intercellular adhesion molecule 1 antibody
        • Major group rhinovirus receptor antibody
        • MALA 2 antibody
        • MALA2 antibody
        • MyD 10 antibody
        • MyD10 antibody
        • P3.58 antibody
        • Surface antigen of activated B cells antibody
        • Surface antigen of activated B cells, BB2 antibody
        see all

      画像

      • Western blot - Anti-ICAM1 antibody [15.2] (ab20)
        Western blot - Anti-ICAM1 antibody [15.2] (ab20)
        All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml

        Lane 1 : SK N BE (Human neuroblastoma) whole cell lysate
        Lane 2 : Saos-2 (Human epithelial-like osteosarcoma cell line) whole cell lysate
        Lane 3 : HT1080 (Human fibrosarcoma) whole cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Predicted band size: 58 kDa
        Observed band size: 58 kDa

      参考文献 (25)

      ab20 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab20 は 25 報の論文で使用されています。

      • Ma L  et al. Agrin Influences Botulinum Neurotoxin A-Induced Nerve Sprouting via miR-144-agrin-MuSK Signaling. Front Cell Dev Biol 8:15 (2020). PubMed: 32083076
      • Bernabeu M  et al. Binding Heterogeneity of Plasmodium falciparum to Engineered 3D Brain Microvessels Is Mediated by EPCR and ICAM-1. mBio 10:N/A (2019). PubMed: 31138740
      • Wu D  et al. The Effects of the CXCR4 Antagonist, AMD3465, on Human Retinal Vascular Endothelial Cells (hRVECs) in a High Glucose Model of Diabetic Retinopathy. Med Sci Monit 25:6946-6954 (2019). PubMed: 31860633
      • Iwasaki O  et al. Involvement of condensin in cellular senescence through gene regulation and compartmental reorganization. Nat Commun 10:5688 (2019). PubMed: 31831736
      • Xu L  et al. Peroxisome Proliferator-Activated Receptor-? Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p. PPAR Res 2019:2715176 (2019). PubMed: 31354796
      View all Publications for this product

      レビューと Q&A

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      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ICAM1 antibody [15.2]

       Poor
      Abreviews
      This product is known to not work in this application or species.
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Urothelium, Bladder Cancer)
      Specification
      Urothelium, Bladder Cancer
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated
      Read More

      Mr. Rudolf Jung

      Verified customer

      投稿 Aug 22 2006

      Question

      We have informed that the product ab27536, which was discontinued. We have been received the information given by you, but I contacted with the customer and she told me she need the antibody is able to recognize the ICAM1 protein intra- and extracellular in samples extracted from human. I would like you may confirm these antibodies recommended by you could work in this condition. Additionally, in the Abcam webpage, I found three antibodies, which are shown below: Anti-ICAM1 antibody [MEM-111] ab2213 Anti-ICAM1 antibody [YN1/1.7.4] ab25375 Anti-ICAM1 antibody (Biotin) ab7815 Which could these ones the differences with the others? Thanks for your support

      Read More
      Answer

      Thank you for the response.

      Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore any single monoclonal antibody will only recognize a region within a single domain and not stain both the intracellular and extracellular region of the target protein.

      Polyclonal antibodies are created by many different immune cells and will contain multiple epitope recognition sites. Although the amount of antibody specific to a particular epitope will vary from lot-to-lot, it is possible for a polyclonal to therefore contain antibodies which will recognize different domains.

      I have reviewed the antibodies which you have listed as well as other polyclonals in our catalog to try and find a product which might be able to recognize the intracellular and extracellular regions.

      ab2213Anti-ICAM1 antibody [MEM-111]: This monoclonal antibody was created with an immunogen from a whole tissue / cell preparation (Human). We have not mapped the exact location of the epitope. Therefore the epitope recognized might be intracellular, transmembrane or extracellular.

      ab25375Anti-ICAM1 antibody [YN1/1.7.4]: This product is also monoclonal.The details of the immunogen for this antibody are not available. However as a monoclonal, this product will not be able to recognize sites in multiple domains.

      ab7815Anti-ICAM1 antibody (Biotin): This product is a rabbit polyclonal and has been raised against the whole protein. As such it is possible that the product will contain antibodies against both extracellular and intracellular regions. We do not test for this type of response and cannot guarantee that the productwill recognizeboth domains. Please be aware that this product is also biotin labeled.

      Our other rabbit polyclonal antibodies were each created from recombinant fragments and would also not be able to recognize both extracellular and intracellular domains.

      I hope this information is helpful. Please do not hesitate to contact me with further questions.

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      Question

      Are all antibodies 2952 - 2955 of the IgG1 subclass? This is important for us to chose the right secondary antibody - we are considering IgG1 PerCP from Becton Dickinson. Are you aware of a different red-shifted secondary antibody one could use with 488 laser excitation? Thanks a lot, kind regards, Ulf Steller Janssen Research Foundation Belgium

      Read More
      Answer

      Yes, all abs 2952-2955 are IgG1. Abcam has a FITC conjugated sheep anti-mouse IgG secondary, product code ab11-500, which may be suitable for your needs.

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