Anti-Iba1 抗体 [EPR6136(2)] - BSA and Azide free (ab221933)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6136(2)] to Iba1 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free
Iba1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6136(2)] to Iba1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WB, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: THP-1 cell lysate. Flow Cyt (Intra): K-562 cells. ICC/IF: Jurkat cells. IHC-P: Human kidney, Human tonsil, human glioma and Human cerebrum tissues.
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特記事項
ab221933 is the carrier-free version of ab178680.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6136(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab221933の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. -
組織特異性
Detected in T-lymphocytes and peripheral blood mononuclear cells. -
配列類似性
Contains 2 EF-hand domains. -
翻訳後修飾
Phosphorylated on serine residues. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. - Information by UniProt
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参照データベース
- Entrez Gene: 199 Human
- Omim: 601833 Human
- SwissProt: P55008 Human
- Unigene: 76364 Human
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別名
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
see all
画像
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This data was developed using ab178680, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Paraffin-embedded sections Human tonsil tissue labelling Iba1 with ab178680 at 1/100000 dilution, followed by a ready to use secondary LeicaDS9800 (BondTM Polymer Refine Detection). Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (BondTM Polymer Refine Detection).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on human tonsil.
The section was incubated with ab178680 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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This data was developed using ab178680, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Paraffin-embedded sections Human glioma tissue labelling Iba1 with ab178680 at 1/100000 dilution, followed by a ready to use secondary LeicaDS9800 (BondTM Polymer Refine Detection). Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (BondTM Polymer Refine Detection).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on human glioma.
The section was incubated with ab178680 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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This data was developed using ab178680, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Paraffin-embedded sections Human cerebrum tissue labelling Iba1 with ab178680 at 1/100000 dilution, followed by a ready to use secondary LeicaDS9800 (BondTM Polymer Refine Detection). Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (BondTM Polymer Refine Detection).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on human cerebrum.
The section was incubated with ab178680 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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This data was developed using ab178680, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Iba1 with purified ab178680 at 1:100 dilution (9.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
Anti-Iba1 antibody [EPR6136(2)] (ab178680) at 1/1000 dilution (Purified) + THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?This data was developed using ab178680, the same antibody clone in a different buffer formulation.
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This data was developed using ab178680, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Iba1 with purified ab178680 at 1:16000 (0.06 µg/ml). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. -
This data was developed using ab178680, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling Iba1 with purified ab178680 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).
プロトコール
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab221933 は 2 報の論文で使用されています。
- Zhang J et al. Human Microglia Extensively Reconstitute in Humanized-BLT Mice With Human Interleukin-34 Transgene and Support HIV-1 Brain Infection. Front Immunol 12:672415 (2021). PubMed: 34093573
- Wassink G et al. Partial white and grey matter protection with prolonged infusion of recombinant human erythropoietin after asphyxia in preterm fetal sheep. J Cereb Blood Flow Metab N/A:N/A (2016). IHC . PubMed: 27207167