Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain, hippocampus)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain, hippocampus
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: .3% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tx
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor 647-conjugated Donkey Anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested in IHC with perfusion fixed frozen sections (IHC-FoFr) and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Collin Gagne
Verified customer
投稿 Jun 21 2019