Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Hippocampus)
Permeabilization
Yes - 0.2% Triton in PBS
Specification
Hippocampus
Blocking step
1% BSA + 5% Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.2% Triton in PBS with 1% BSA
Dilution
1/100
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Abcam response
Thank you for sharing this data with us. ab178847 has not previously been tested in IHC on frozen sections and based on this Abreview, it looks like this antibody does not work in this application, under these conditions. Abcam would welcome feedback from other customers (www.abcam.com/abtrial).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Sergi Bayod
Verified customer
投稿 Mar 03 2017