Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus monkey Tissue sections (Brain, Cerebrum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 high pH
Permeabilization
No
Specification
Brain, Cerebrum
Fixative
Formaldehyde
Other product details
Incubation time
32 minute(s) · Temperature: 37°C · Diluent: Life Technologies Antibody Diluent RTU
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Ventana Omni Map anti Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
IHC staining was performed on a Ventana Discovery XT automated staining platform. Anitgen retrieval performed on the stainer at 95°C on mild CC1 setting. Chromomap DAB Kit was used to visualize target antigen. Ventana Hematoxylin 1 and Bluing Reagent were used for counterstain.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
David Weil
Verified customer
投稿 Sep 05 2018