Anti-Iba1 抗体 (ab5076)
Key features and details
- Goat polyclonal to Iba1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Iba1 antibody
Iba1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Iba1 -
由来種
Goat -
特異性
ab5076 is expected to recognize the isoforms represented by NP_001614 and NP_116573 but not NP_004838.
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アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Pig, Macaque monkey
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免疫原
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ポジティブ･コントロール
- WB: Human lymph node and rat brain tissue lysate. IHC-P: Human spleen and rat brain tissue.
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特記事項
The Iba1 antibody (ab5076) is commonly used as a marker of microglia activation in staining and immunohistochemistry, given that ionized calcium binding adaptor molecule 1 (Iba1) is a microglia/macrophage-specific calcium-binding protein with actin-bundling activity that participates in membrane ruffling and phagocytosis in activated microglia.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5076の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (27) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (7) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 16-17 kDa (predicted molecular weight: 17 kDa).
A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background. |
| 特記事項 |
|---|
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 16-17 kDa (predicted molecular weight: 17 kDa). A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background. |
ターゲット情報
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機能
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. -
組織特異性
Detected in T-lymphocytes and peripheral blood mononuclear cells. -
配列類似性
Contains 2 EF-hand domains. -
翻訳後修飾
Phosphorylated on serine residues. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. - Information by UniProt
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参照データベース
- Entrez Gene: 199 Human
- Entrez Gene: 397271 Pig
- Entrez Gene: 29427 Rat
- Omim: 601833 Human
- SwissProt: P55008 Human
- SwissProt: P81076 Pig
- SwissProt: P55009 Rat
- Unigene: 76364 Human
see all -
別名
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
see all
画像
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Western blot - Anti-Iba1 antibody (ab5076)All lanes : Anti-Iba1 antibody (ab5076) at 1 µg/ml
Lane 1 : Human lymph node tissue lysate in RIPA buffer
Lane 2 : Rat brain tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 17 kDaDetected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)Immunohistochemistry analysis of paraffin-embedded human spleen tissue labeling Iba1 with ab5076 at 5 µg/mL (left). Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody (right). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)Image from Iris Margalit Trutzer et al, ACTA neuropathologica communications, 7, 40, Fig. 7; doi: 10.1186/s40478-019-0684-8Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lateral prefrontal cortex tissue labeling iba1 labeled microglia (labeled with black arrows) within the cortex using ab5076 at 1/1000 dilution. (Cropped image).
Sections were rinsed in 0.01 m PBS, pH 7.4, followed by 10% serum matching the species of the secondary antibody, 5% bovine serum albumin, and 0.1% Triton X-100 in 0.01 m PBS blocking solution for 1 h and incubated for 2 days at 4 °C in primary antibody. The sections were rinsed in PBS and incubated overnight at 4 °C with a horse anti-Goat IgG (Biotinylated) secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)IHC-P image of Iba1 staining on rat brain sections using ab5076 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab5076 was diluted 1:1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was Rabbit polyclonal to anti sheep/goat IgG conjugated to biotin (1:200)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (812)
ab5076 は 812 報の論文で使用されています。
- Keane L et al. mTOR-dependent translation amplifies microglia priming in aging mice. J Clin Invest 131:N/A (2021). IHC-P ; Mouse . PubMed: 33108356
- Pang N et al. Transcranial Ultrasound Stimulation of Hypothalamus in Aging Mice. IEEE Trans Ultrason Ferroelectr Freq Control 68:29-37 (2021). PubMed: 31985418
- Chiazza F et al. The Stroke-Induced Increase of Somatostatin-Expressing Neurons is Inhibited by Diabetes: A Potential Mechanism at the Basis of Impaired Stroke Recovery. Cell Mol Neurobiol 41:591-603 (2021). PubMed: 32447613
- Ma J et al. EphA1 Activation Induces Neuropathological Changes in a Mouse Model of Parkinson's Disease Through the CXCL12/CXCR4 Signaling Pathway. Mol Neurobiol 58:913-925 (2021). PubMed: 33057926
- Sinha P et al. Trehalose Ameliorates Seizure Susceptibility in Lafora Disease Mouse Models by Suppressing Neuroinflammation and Endoplasmic Reticulum Stress. Mol Neurobiol 58:1088-1101 (2021). PubMed: 33094475