Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (jejunum)
Specification
jejunum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Other product details

Dilution
1/300
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/Azide

Secondary antibody

Name
Non-Abcam antibody was used: rabbit anti goat
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
In adult human pwax jejunum pwax section this Ab reagent seems to be excellent for demonstrating macrophages.
I used a streptAvidin-peroxidase system with DAB as chromogen.

Mr. Carl Hobbs

Verified customer

投稿 Dec 17 2007

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