Key features and details
- Goat polyclonal to Iba1
- Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Mouse, Rabbit, Human
- Isotype: IgG
Iba1 一次抗体 製品一覧
製品の詳細Goat polyclonal to Iba1
This antibody is expected to recognise P55008-1 or isoform 3 (NP_001614.3) only. This antibody gave excellent staining in paraffin embedded human adult tissue and embyronic rat tissue. For WB, we recommend blocking in 3-5% milk, NOT BSA. Blocking in BSA gives high background. Primary incubation 1 hour at room temperature.
アプリケーション適用あり: WB, Flow Cyt, ICC/IF, IHC-Pmore details
種交差性交差種: Mouse, Rabbit, Human
交差が予測される動物種: Cow, Pig, Macaque monkey
- WB: Recombinant Human Iba1 protein (ab117478), Human peripheral blood mononucleocyte and frontal cortex lysate. IHC-P: Mouse brain; Human placenta and jejunum; rat embryonic liver tissue. ICC/IF: Caco-2 cells Flow Cyt: K562 cells
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab48004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
We recommend blocking in 3-5% milk, not BSA. Blocking in BSA gives high background in WB.
Primary incubation 1 hour at room temperature.
|Flow Cyt||Use a concentration of 10 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
機能Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
組織特異性Detected in T-lymphocytes and peripheral blood mononuclear cells.
配列類似性Contains 2 EF-hand domains.
翻訳後修飾Phosphorylated on serine residues.
細胞内局在Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
- Information by UniProt
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
Immunocytochemistry/ Immunofluorescence analysis of Caco-2 cells labeling Iba1 with ab48004 at 10µg/mL. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 secondary antibody at 2µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
ab48004 at 1/300 staining human jejunum tissue sections by IHC-P. A heat mediated antigen retrieval step in citric acid buffer was performed and the tissue was blocked in BSA before incubation with the antibody for 2 hours. A biotinylated rabbit anti-goat antibody was used as the secondary.
ab48004 diluted 1/200 with TBS/BSA/Azide staining rat d15 embryonic liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat-mediated antigen retrieval step in citric acid buffer at pH6 was performed prior to blocking with 1% BSA for 10 minutes at room temperature. Incubation time with the primary antibody was 16 minutes. A biotinylated rabbit anti-goat was used as the secondary.
Anti-Iba1 antibody (ab48004) at 0.5 µg/ml + human frontal cortex lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 17 kDa
Primary incubation was 1 hour
Paraffin embedded mouse brain tissue stained for Iba1 with ab48004 (2 µg/ml) in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry analysis of K562 cells labeling Iba1 with ab48004 at 10µg/mL (blue). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. An Alexa Fluor 488 secondary antibody at 1µg/mL was used as the secondary antibody.
IgG control: Unimmunized goat IgG (black) followed by Alexa Fluor 488 secondary antibody.
ab48004 は 12 報の論文で使用されています。
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