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    iba1-antibody-ab108539.pdf

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Anti-Iba1 抗体 (ab108539)

  • Datasheet
  • SDS
Reviews (3)Q&A (6)References (31)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
  • Western blot - Anti-Iba1 antibody (ab108539)
  • Western blot - Anti-Iba1 antibody (ab108539)

Key features and details

  • Rabbit polyclonal to Iba1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Iba1 antibody [EPR16588] (ab178846)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産
医薬用外毒物

製品の概要

  • 製品名

    Anti-Iba1 antibody
    Iba1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Iba1
  • 由来種

    Rabbit
  • 特異性

    In WB, we recommend blocking in milk. Blocking with BSA gives high background. If looking for a monoclonal anti-Iba1 alternative we can recommend our RabMAb ab178846
  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Iba1 (C terminal).
    Database link: P55008

  • ポジティブ・コントロール

    • Iba1 (Human) full length recombinant protein; Rat brain lysate; Human fetal lymphocytes; spleen tissue lysate; THP1, C6, NR8383 whole cell lysate
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Immunology
    • Immune Interventions
    • Transplantation
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Neuroscience
    • Development

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Iba1 protein (ab105593)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108539の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/1000. Predicted molecular weight: 17 kDa.

We recommend blocking in 3-5% milk. Blocking with BSA gives high background.

IHC-P (3)
1/100 - 1/500.
特記事項
WB
1/500 - 1/1000. Predicted molecular weight: 17 kDa.

We recommend blocking in 3-5% milk. Blocking with BSA gives high background.

IHC-P
1/100 - 1/500.

ターゲット情報

  • 機能

    Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
  • 組織特異性

    Detected in T-lymphocytes and peripheral blood mononuclear cells.
  • 配列類似性

    Contains 2 EF-hand domains.
  • 翻訳後修飾

    Phosphorylated on serine residues.
  • 細胞内局在

    Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
  • Target information above from: UniProt accession P55008 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 199 Human
    • Entrez Gene: 11629 Mouse
    • Entrez Gene: 29427 Rat
    • Omim: 601833 Human
    • SwissProt: P55008 Human
    • SwissProt: O70200 Mouse
    • SwissProt: P55009 Rat
    • Unigene: 76364 Human
    • Unigene: 10747 Mouse
    • Unigene: 32080 Rat
    see all
  • 別名

    • AIF 1 antibody
    • AIF-1 antibody
    • Aif1 antibody
    • AIF1 protein antibody
    • AIF1_HUMAN antibody
    • Allograft inflammatory factor 1 antibody
    • Allograft inflammatory factor 1 splice variant G antibody
    • allograft inflammatory factor-1 splice variant Hara-1 antibody
    • balloon angioplasty responsive transcription antibody
    • BART 1 antibody
    • G1 antibody
    • G1 putative splice variant of allograft inflamatory factor 1 antibody
    • IBA 1 antibody
    • IBA1 antibody
    • interferon gamma responsive transcript antibody
    • Interferon responsive transcript 1 antibody
    • interferon responsive transcript factor 1 antibody
    • Ionized calcium binding adapter molecule 1 antibody
    • Ionized calcium-binding adapter molecule 1 antibody
    • ionized calcium-binding adapter molecule antibody
    • IRT 1 antibody
    • IRT1 antibody
    • Microglia response factor antibody
    • MRF1 antibody
    • Protein g1 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)

    IHC image of Iba1 staining in normal mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)

    IHC image of Iba1 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)

    IHC image of Iba1 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab108539)
    ab108539, at 1/100 dilution, staining Iba1 in formalin-fixed, paraffin-embedded Human fetal lymphocytes by Immunohistochemistry.
  • Western blot - Anti-Iba1 antibody (ab108539)
    Western blot - Anti-Iba1 antibody (ab108539)
    All lanes : Anti-Iba1 antibody (ab108539) at 1/1000 dilution

    Lane 1 : Human Iba1 recombinant protein at 0.1 µg
    Lane 2 : HEK293 whole cell lysate at 20 µg
    Lane 3 : A431 whole cell lysate at 20 µg
    Lane 4 : NIH3T3 whole cell lysate at 30 µg
    Lane 5 : Human spleen tissue lysate at 20 µg
    Lane 6 : Mouse spleen tissue lysate at 30 µg
    Lane 7 : Rat spleen tissue lysate at 30 µg
    Lane 8 : U937 whole cell lysate at 30 µg
    Lane 9 : MOLT4 whole cell lysate at 20 µg
    Lane 10 : THP1 whole cell lysate at 30 µg
    Lane 11 : THP1 whole cell lysate, PMA treated at 30 µg
    Lane 12 : Raw 264.7 whole cell lysate at 30 µg
    Lane 13 : C6 whole cell lysate at 30 µg
    Lane 14 : NR8383 whole cell lysate at 30 µg
    Lane 15 : NIH3T3 whole cell lysate - BLOCKED IN 5% BSA at 30 µg
    Lane 16 : Human spleen tissue lysate - BLOCKED IN 5% BSA at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa


    Exposure time: 3 minutes


    Lanes 1-14: Blocked in 3% milk for 1 hour (RT). Lanes 15-16: Blocked in 5% BSA for 1 hour (RT).

    Abcam recommends blocking in milk for cleaner blots with reduced background, in comparison to BSA. 

    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab108539 (anti-Iba1 antibody; 1/1000) for 18 hours at 4°C. Antibody binding was detected using HRP-labelled anti-Rabbit IgG for 1 hour at room temperature and visualised using ECL development solution ab133406.

  • Western blot - Anti-Iba1 antibody (ab108539)
    Western blot - Anti-Iba1 antibody (ab108539)
    Anti-Iba1 antibody (ab108539) at 1/500 dilution + Rat brain lysate

    Predicted band size: 17 kDa

プロトコール

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (31)

ab108539 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108539 は 31 報の論文で使用されています。

  • Petrovic A  et al. Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica. Front Cell Neurosci 15:661492 (2021). PubMed: 33815068
  • El-Derany MO  et al. Bone Marrow-Derived Mesenchymal Stem Cells Reverse Radiotherapy-Induced Premature Ovarian Failure: Emphasis on Signal Integration of TGF-ß, Wnt/ß-Catenin and Hippo Pathways. Stem Cell Rev Rep 17:1429-1445 (2021). PubMed: 33594662
  • Boroujeni ME  et al. Inflammatory Response Leads to Neuronal Death in Human Post-Mortem Cerebral Cortex in Patients with COVID-19. ACS Chem Neurosci 12:2143-2150 (2021). PubMed: 34100287
  • Giridharan VV  et al. Neuroinflammation trajectories precede cognitive impairment after experimental meningitis-evidence from an in vivo PET study. J Neuroinflammation 17:5 (2020). PubMed: 31901235
  • Sayd A  et al. Depletion of brain perivascular macrophages regulates acute restraint stress-induced neuroinflammation and oxidative/nitrosative stress in rat frontal cortex. Eur Neuropsychopharmacol 34:50-64 (2020). PubMed: 32245674
View all Publications for this product

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1-9 of 9 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: MW pH9
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1500µg/mL
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Gaia Brezzo

Verified customer

投稿 Dec 16 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

投稿 Dec 01 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

投稿 Nov 30 2015

Question

I bought your company's antibody anti Iba1(ab108539) for western blotting, but there are three band, their molecular weight are 17kd, 34kd and 75kd. The main band is 34kd, is not 17kd. Would you like to help me to figure out the situation.
There is DTT in lysis buffer, there is reducing reagent in denature buffer.

Read More

Abcam community

Verified customer

Asked on Mar 27 2012

Answer

Thank you for contacting Abcam.

So that I can better help you with this, would you be able to provide me with a copy of the image that you are getting and also the protocol that you are using, in particular could you include the following information:

1 - What sample type are you using?

2 - How much sample are you loading?

3- What type of blocking agent are you using?

4- What primary antibody concentrations have you tried and how long do you incubate for?

5 - How long do you boil your samples for?

I look forward to your reply and helping you resolve this issue.

Read More

Abcam Scientific Support

Answered on Mar 28 2012

Question

Does "rabbit polyclonal antibody to Iba-1" (ab108539) is useful for visualization of microglial cells of mouse brain? I haven't seen any application about this type of cell in the datasheet form.
Yours Sincerely;

Read More

Abcam community

Verified customer

Asked on Aug 10 2012

Answer

Thank you for contacting us.

I can confirm that antibody ab108539 could be used for detection of Iba1 in microglia cells of mouse brain. This assumption is however based on the expression of this protein in mouse microglia cells; we unfortunately haven't tried this in our lab however it would work.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Aug 10 2012

Question

Dear All,

I am forwarding you the email / complain from our customer. These items are on the invoice 1330583.

We received the goods and good condition and delivered direct in the same day.

Please provide the necessary action.

Thanking you in advance!

With Best Regards,

I have very good experience with ABCAM antibodies. However, today I am reporting to you the results of testing three antibodies that you just supplied us from ABCAM. We stained sections of rat’s brain (two times) with different dilutions of the antibodies using ACB method of immunocytochemistry.

1) ab15690: Mouse monoclonal [1022-5] to Iba1. Negative results with the following dilutions, 1:200, 1:500, 1000, 1,2000, 1:5000 and 1: 10,1000.

2) ab108539 Rabbit polyclonal to Iba1: It has worked with best dilution 1:500-1:1000. This meant that the antibody is not very economic as we need high concentration (1:50-1:100) of the antibodies when we need to use it for fluorescent immunocytochemistry. In comparison, your Goat anti Iba1 (ab5076) which we bought last year has produced very good results with 1:5000 dilution for peroxidase ABC method and 1:500 dilution for fluorescent.

3) ab7260 Rabbit polyclonal to GFAP - Astrocyte Marker;

It has worked with 1:5000 dilution but the staining was not that great compared for example with Vector mouse anti-GFAP antibody!

I hope that this is of help.

Best regards.

Read More

Abcam community

Verified customer

Asked on Jun 12 2012

Answer

Thank you for taking the time to contact us. I am sorry to hearthe customer hashad difficulty obtaining satisfactory results from these threeantibodies.

I would like to reassure you that in the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide images which would help us to assess the results.

Please note Ihave sent the questionnaire for IHC and ICC. If the customer has used another application, please let me know and I can send the correct form so we can obtain the relevant information required.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.



Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results

Read More

Abcam Scientific Support

Answered on Jun 12 2012

Question

Dear xxxx, Just to let you know I have taken delivery of the new lba-1 antibody (polyclonal) you so kindly sent me. Thank you for my surprize calendar 2012 and the related paperwork, which I didn’t receive the first time. Just to let you know, I always wanted your mini calendars, now that I have one, I am jolly pleased. I will get working on the antibody on Monday. Thank you so much for everything.

Read More

Abcam community

Verified customer

Asked on Dec 09 2011

Answer

Here is a quite detailed protocol for performing paraffin embedded immunohistochemical staining, which has the buffer composition for Tris-EDTA pH 9 which I suggested trying: https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf Tris-EDTA buffer: 10 mM Tris Base, 1 mM EDTA, 0.05% Tween 20, pH9.0 Its quite a useful guide, especially when troubleshooting for a new experiment. I hope it is of help to you. Have a nice weekend.

Read More

Abcam Scientific Support

Answered on Dec 09 2011

Question

Phone call requesting futher information of the protocol used with this antibody in order to imporove the staining currently observed.

Read More

Abcam community

Verified customer

Asked on Dec 07 2011

Answer

Thank you for contacting us again. As discussed over the phone, I have arranged for the free of charge replacement with ab5076 to be delivered to you at the following address (taken from your previous order): The new order number is: This should be with you tomorrow. I hope this one works better for you. Although your collegues have said that they have not required any antigen retrieval, several of our customers have used citrate mediated antigen retrieval so you may need to optimise when using your particular samples. I was also wrong to say that this antibody has not yet been tested in mouse samples, several of our customers have used it for IHC with mouse tissue and I will amend this information on the datasheet. As discussed I have also attached a questionnaire which it would be very helpful if you could fill out. This will allow me to investigate this case internally and initiate any additional testing where necessary. I look forward to receiving your reply.

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Abcam Scientific Support

Answered on Dec 07 2011

Question

Good morning, I would like to know which of the following antibodies works better for inmunohistochemistry in parafin embeded tissues.... Rabbit Polyclonal anti-Iba-1 Cod. Ab108539   Goat Polyclonal anti-Iba-1 Cod. Ab5076   donkey anti-goat Cod. Ab6884 Please, let me know asap... thank you ! Best regards,

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Abcam community

Verified customer

Asked on Sep 26 2011

Answer

Thank you for your enquiry. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Iba1 antibody tested in the species and application you are using should be suitable for your applications. I can confirm that both ab5076 and ab108539 have been tested and guaranteed for IHC-P. Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet). Regarding the secondary antibody, ab6884 Donkey polyclonal Secondary Antibody to Goat IgG (Biotin) is tested and guaranteed in IHC-P. As an anti-goat antibody, it would be suitable for use with Anti-Iba1 antibody (ab5076), as this primary antibody is raised in goat. With regards to ab108539 Anti-Iba1 antibody, this is a rabbit polyclonal, and therefore requires an anti-rabbit secondary antibody and ab6884 would not be a suitable secondary to use with ab108539.. I hope this information will be helpful to you. Should you have any further questions regarding your search and selection of products, please do not hesitate to ask.

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Abcam Scientific Support

Answered on Sep 26 2011

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