Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% normal rabbit serum
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Rabbit Anti-Goat IgG Antibody (H+L), Biotinylated
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Abcam response
We would like to thank the reviewer for submitting this AbReview. As the product has not been validated with human samples, it may not be effective in this application and further optimization may improve results. We appreciate the time and effort that the reviewer has put in to testing this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 07 2020