Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Monkey Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.2% triton
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: dk sérum 5%
Dilution
1/250
Secondary antibody
Dilution
1/250
Name
Abcam antibody:Donkey Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed
Additional data
Additional Notes
We also test with higher concentration of triton (2.5%) and with antigene retrieval method (ac/eth) : no more results
Abcam response
Abcam has not tested ab107159 in Moneky samples and thus can't say how it should have performed. This individual received a credit for testing this antibody in Moneky samples as part of our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Francoise Geffroy
Verified customer
投稿 Mar 07 2019