Key features and details
- Goat polyclonal to Iba1
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
- 倫理基準に準拠 - アニマル・フリーの生産
Iba1 一次抗体 製品一覧
製品の詳細Goat polyclonal to Iba1
特異性If looking for a monoclonal anti-Iba1 alternative we can recommend our RabMAb ab178846. This has the same immunogen as ab5076. In recent testing in IHC-P this antibody only worked in Rat tissue, therefore we have removed Human and mouse as a "does not react with" species. However, feedback from our customers and publications suggest that this antibody recognizes human and mouse. Nevertheless, we cannot guarantee this antibody in these species.
アプリケーション適用あり: IHC-Pmore details
交差が予測される動物種: Dog, Pig, Macaque monkey, Chinese hamster, Common marmoset
Synthetic peptide corresponding to Rat Iba1 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
Concentration information loading...
精製度Immunogen affinity purified
機能Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
組織特異性Detected in T-lymphocytes and peripheral blood mononuclear cells.
配列類似性Contains 2 EF-hand domains.
翻訳後修飾Phosphorylated on serine residues.
細胞内局在Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
- Information by UniProt
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
IHC image of Iba1 staining in a section of formalin-fixed paraffin-embedded normal rat brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab107159, 5ug/ml, for 15 mins at room temperature. An HRP-conjugated donkey anti-goat IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
IHC-P image of Iba1 staining on marmoset (common) kidney sections using ab107159 (1:5000). The image shows macrophage infiltration in the cortex. The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 16 hours. The secondary antibody was rabbit polyclonal to sheep IgG conjugated to biotin at 1:200.
ab107159 staining Iba1 in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted HRP-conjugated anti-goat IgG polyclonal was used as the secondary antibody. Kupffer cells are positive for Iba-1.
IHC-P image of Iba1 staining on rat spleen sections using ab107159 (1:2000). The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours. The secondary antibody was goat polyclonal to anti-sheep conjugated to biotin at 1:250.
ab107159 は 114 報の論文で使用されています。
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