Anti-Iba1 抗体 [1022-5] (ab15690)

Reviews (15)Q&A (26)References (90)

Key features and details

  • Mouse monoclonal [1022-5] to Iba1
  • Suitable for: IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-Iba1 antibody [1022-5]
    Iba1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [1022-5] to Iba1

  • 由来種

    Mouse

  • 特異性

    Some customers have reported successful staining using this antibody on frozen sections, however ab15690 is not routinely tested on frozen sections and we do not recommend this antibody for use with frozen tissue. 

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: IHC-Fr

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Recombinant full length protein corresponding to Human Iba1.
    Database link: P55008

  • ポジティブ･コントロール

    • IHC-P: Human spleen. ICC/IF: Human induced microglia.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for IHC-Fr.

    • ターゲット情報

    • 機能

      Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

    • 組織特異性

      Detected in T-lymphocytes and peripheral blood mononuclear cells.

    • 配列類似性

      Contains 2 EF-hand domains.

    • 翻訳後修飾

      Phosphorylated on serine residues.

    • 細胞内局在

      Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
    • Information by UniProt

    • 参照データベース

      • 別名

        • AIF 1 antibody
        • AIF-1 antibody
        • Aif1 antibody
        • AIF1 protein antibody
        • AIF1_HUMAN antibody
        • Allograft inflammatory factor 1 antibody
        • Allograft inflammatory factor 1 splice variant G antibody
        • allograft inflammatory factor-1 splice variant Hara-1 antibody
        • balloon angioplasty responsive transcription antibody
        • BART 1 antibody
        • G1 antibody
        • G1 putative splice variant of allograft inflamatory factor 1 antibody
        • IBA 1 antibody
        • IBA1 antibody
        • interferon gamma responsive transcript antibody
        • Interferon responsive transcript 1 antibody
        • interferon responsive transcript factor 1 antibody
        • Ionized calcium binding adapter molecule 1 antibody
        • Ionized calcium-binding adapter molecule 1 antibody
        • ionized calcium-binding adapter molecule antibody
        • IRT 1 antibody
        • IRT1 antibody
        • Microglia response factor antibody
        • MRF1 antibody
        • Protein g1 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)

        IHC image of Iba1 staining in human normal spleen formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15690, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

         

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

        *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)

        Immunohistochemical analysis of paraffin embedded human spleen sections, labelling Iba1 with ab15690.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)

        Immunohistochemical analysis of paraffin embedded human spleen sections, labelling Iba1 with ab15690.

      参考文献

      ab15690 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab15690 は 90 報の論文で使用されています。

      • Amal H  et al. S-nitrosylation of E3 ubiquitin-protein ligase RNF213 alters non-canonical Wnt/Ca+2 signaling in the P301S mouse model of tauopathy. Transl Psychiatry 9:44 (2019). PubMed: 30696811
      • Hu JZ  et al. Silencing of lncRNA PKIA-AS1 Attenuates Spinal Nerve Ligation-Induced Neuropathic Pain Through Epigenetic Downregulation of CDK6 Expression. Front Cell Neurosci 13:50 (2019). PubMed: 30873006
      • Guo BJ  et al. Andrographolide derivative ameliorates dextran sulfate sodium-induced experimental colitis in mice. Biochem Pharmacol 163:416-424 (2019). PubMed: 30878550
      • Tao T  et al. Modulating cAMP responsive element binding protein 1 attenuates functional and behavioural deficits in rat model of neuropathic pain. Eur Rev Med Pharmacol Sci 23:2602-2611 (2019). PubMed: 30964189
      • Klotz D & Gerhauser I Interferon-Stimulated Genes-Mediators of the Innate Immune Response during Canine Distemper Virus Infection. Int J Mol Sci 20:N/A (2019). PubMed: 30939763
      View all Publications for this product

      レビューと Q&A

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      1-10 of 41 Abreviews or Q&A

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Iba1 antibody [1022-5] - Microglial marker

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Rat Tissue sections (brain sections)
      Specification
      brain sections
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 37°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      投稿 Sep 12 2013

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [1022-5]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rabbit Tissue sections (Brain (rabbit neonate))
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
      Permeabilization
      No
      Specification
      Brain (rabbit neonate)
      Blocking step
      (agent) for 10 minute(s) · Concentration: 0.5% · Temperature: 24°C
      Fixative
      Formaldehyde
      Read More

      Rebeca Figueira

      Verified customer

      投稿 Aug 13 2019

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Iba1 antibody [1022-5]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Microglia)
      Permeabilization
      Yes - 0.3% Triton X-100
      Specification
      Microglia
      Blocking step
      (agent) for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Apr 11 2019

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Iba1 antibody [1022-5]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Rat Tissue sections (Spinal cord)
      Antigen retrieval step
      None
      Permeabilization
      Yes - PBS + 0.2% triton + 4% BSA
      Specification
      Spinal cord
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Dr. Alexandre Magno

      Verified customer

      投稿 Jul 20 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [1022-5]

       Below Average
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rhesus monkey Tissue sections (retina)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate pH 6
      Permeabilization
      No
      Specification
      retina
      Blocking step
      Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Mar 26 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [1022-5] - Microglial marker

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
      Sample
      Pig Cell (Brain)
      Specification
      Brain
      Permeabilization
      Yes - Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% Tween 20, pH 6.0) and a pressure cooker Temp 91-99C.
      Fixative
      Formaldehyde
      Read More

      Shadi Malaeb

      Verified customer

      投稿 Mar 25 2014

      Question

      Hoping you'll be able to help me with something, I recently purchased an IBA1 antibody (ab15690). It is 100ug at a 3.1 mg/ml concentration. According to the datasheet this antibody does not need to be reconstituted but I would prefer to have a larger amount for ease of aliquoting and using etc.and also a conc. of 3.1mg/ml seems very high. Would it be ok to make the antibody up to 1ml (with 10mM PBS pH7.4) to achieve a dilution of 100ug/ml? Also, I wish to use this antibody for IHC-FrFl on rat brain tissue, would you be able to recommend the dilution factor I should use for this purpose if I am using the new concentration of antibody?

      Read More
      Answer

      This concentration is indeed quite high for an antibody so if you wish to dilute it in PBS then that is fine. I would say, that diluting it down to 1 mg/ml would be fine and is standard for most antibodies. Diluting it down to 100 ug/ml is quite a big dilution and I would not recommend this as it may affect the stability of this product especially when storing it at -20ºC or -80ºC.
      I notice that you are wanting to use this antibody for IHC-FrFl on rat brain tissue. As stated on the datasheet, although some customers have reported successful staining using this antibody on frozen sections, we do not routinely test this antibody on frozen sections, and we do not recommend it for use on these. For this reason, we have not suggested a dilution factor for this application. If you still want to proceed with this experiment I would suggest following the IHC-P guidelines so using 10-20 ug/ml of antibody per experiment.

      Read More

      Western blot abreview for Anti-Iba1 antibody [1022-5] - Microglial marker

       Poor
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      30 µg
      Gel Running Conditions
      Reduced Denaturing (15%)
      Sample
      Rat Cell lysate - whole cell (cortical)
      Specification
      cortical
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      投稿 Oct 07 2013

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [1022-5] - Microglial marker

       Inconclusive
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rabbit Tissue sections (Brain)
      Specification
      Brain
      Fixative
      10% NBF
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
      Permeabilization
      Yes - Proteinase K
      Blocking step
      Serum as blocking agent for 5 minute(s) · Concentration: 0.05% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 08 2013

      Western blot abreview for Anti-Iba1 antibody [1022-5]

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (Brain-microglia)
      Gel Running Conditions
      Reduced Denaturing (12% gel)
      Loading amount
      35 µg
      Specification
      Brain-microglia
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Oct 18 2012

      1-10 of 41 Abreviews or Q&A

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