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    iba1-antibody-1022-5-ab15690.pdf

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Anti-Iba1 抗体 [1022-5] (ab15690)

  • Datasheet
  • SDS
Reviews (15)Q&A (26)References (113)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)

Key features and details

  • Mouse monoclonal [1022-5] to Iba1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

FITC

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human Iba1 protein (ab105593)
Biochemical
Product image
Congo Red, Amyloid fibril-binding dye (ab145645)
一次抗体
Product image
FITC Anti-Iba1 antibody [1022-5] (ab15691)

関連製品

製品の概要

  • 製品名

    Anti-Iba1 antibody [1022-5]
    Iba1 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [1022-5] to Iba1
  • 由来種

    Mouse
  • 特異性

    Some customers have reported successful staining using this antibody on frozen sections, however ab15690 is not routinely tested on frozen sections and we do not recommend this antibody for use with frozen tissue. 

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: IHC-Fr
  • 種交差性

    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human Iba1.
    Database link: P55008

  • ポジティブ・コントロール

    • IHC-P: Human spleen. ICC/IF: Human induced microglia.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.05% KATHON™ CG/ICP
    Constituents: 99% PBS, 0.5% BSA
  • Concentration information loading...
  • 精製度

    Affinity purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    1022-5
  • アイソタイプ

    IgG2b
  • 研究分野

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Immunology
    • Immune Interventions
    • Transplantation
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Neuroscience
    • Development

関連製品

  • Alternative Versions

    • FITC Anti-Iba1 antibody [1022-5] (ab15691)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human Iba1 protein (ab105593)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
    • Anti-CD68 antibody [FA-11] (ab53444)
    • Anti-CD11b antibody [M1/70] (ab8878)
    • Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab15690の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (8)
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
特記事項
IHC-P
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
追加情報
Is unsuitable for IHC-Fr.

ターゲット情報

  • 機能

    Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
  • 組織特異性

    Detected in T-lymphocytes and peripheral blood mononuclear cells.
  • 配列類似性

    Contains 2 EF-hand domains.
  • 翻訳後修飾

    Phosphorylated on serine residues.
  • 細胞内局在

    Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
  • Target information above from: UniProt accession P55008 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 199 Human
    • Omim: 601833 Human
    • SwissProt: P55008 Human
    • Unigene: 76364 Human
    • 別名

      • AIF 1 antibody
      • AIF-1 antibody
      • Aif1 antibody
      • AIF1 protein antibody
      • AIF1_HUMAN antibody
      • Allograft inflammatory factor 1 antibody
      • Allograft inflammatory factor 1 splice variant G antibody
      • allograft inflammatory factor-1 splice variant Hara-1 antibody
      • balloon angioplasty responsive transcription antibody
      • BART 1 antibody
      • G1 antibody
      • G1 putative splice variant of allograft inflamatory factor 1 antibody
      • IBA 1 antibody
      • IBA1 antibody
      • interferon gamma responsive transcript antibody
      • Interferon responsive transcript 1 antibody
      • interferon responsive transcript factor 1 antibody
      • Ionized calcium binding adapter molecule 1 antibody
      • Ionized calcium-binding adapter molecule 1 antibody
      • ionized calcium-binding adapter molecule antibody
      • IRT 1 antibody
      • IRT1 antibody
      • Microglia response factor antibody
      • MRF1 antibody
      • Protein g1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)

      IHC image of Iba1 staining in human normal spleen formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15690, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)

      Immunohistochemical analysis of paraffin embedded human spleen sections, labelling Iba1 with ab15690.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)

      Immunohistochemical analysis of paraffin embedded human spleen sections, labelling Iba1 with ab15690.

    プロトコール

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (113)

    ab15690 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab15690 は 113 報の論文で使用されています。

    • Goebel U  et al. Argon reduces microglial activation and inflammatory cytokine expression in retinal ischemia/reperfusion injury. Neural Regen Res 16:192-198 (2021). PubMed: 32788476
    • Ding H  et al. Elevated intracranial pressure induces IL-1ß and IL-18 overproduction via activation of the NLRP3 inflammasome in microglia of ischemic adult rats. Int J Mol Med 47:183-194 (2021). PubMed: 33236152
    • Zheng Y  et al. Electroacupuncture effects on the P2X4R pathway in microglia regulating the excitability of neurons in the substantia gelatinosa region of rats with spinal nerve ligation. Mol Med Rep 23:N/A (2021). PubMed: 33398365
    • Lee DS & Kim JE Protein disulfide isomerase-mediated S-nitrosylation facilitates surface expression of P2X7 receptor following status epilepticus. J Neuroinflammation 18:14 (2021). PubMed: 33407649
    • Wang YS  et al. HCMV infection and IFITM3 rs12252 are associated with Rasmussen's encephalitis disease progression. Ann Clin Transl Neurol 8:558-570 (2021). PubMed: 33465303
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 41 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Iba1 antibody [1022-5] - Microglial marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brain sections)
    Specification
    brain sections
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 37°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 12 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [1022-5]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Brain (rabbit neonate))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
    Permeabilization
    No
    Specification
    Brain (rabbit neonate)
    Blocking step
    (agent) for 10 minute(s) · Concentration: 0.5% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Rebeca Figueira

    Verified customer

    投稿 Aug 13 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Iba1 antibody [1022-5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Microglia)
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Microglia
    Blocking step
    (agent) for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 11 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Iba1 antibody [1022-5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS + 0.2% triton + 4% BSA
    Specification
    Spinal cord
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    DR. Alexandre Magno

    Verified customer

    投稿 Jul 20 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [1022-5]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (retina)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH 6
    Permeabilization
    No
    Specification
    retina
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 26 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [1022-5] - Microglial marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Pig Cell (Brain)
    Specification
    Brain
    Permeabilization
    Yes - Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% Tween 20, pH 6.0) and a pressure cooker Temp 91-99C.
    Fixative
    Formaldehyde
    Read More

    Shadi Malaeb

    Verified customer

    投稿 Mar 25 2014

    Western blot abreview for Anti-Iba1 antibody [1022-5] - Microglial marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Rat Cell lysate - whole cell (cortical)
    Specification
    cortical
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 07 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [1022-5] - Microglial marker

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Brain)
    Specification
    Brain
    Fixative
    10% NBF
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    Yes - Proteinase K
    Blocking step
    Serum as blocking agent for 5 minute(s) · Concentration: 0.05% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 08 2013

    Western blot abreview for Anti-Iba1 antibody [1022-5]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Brain-microglia)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    35 µg
    Specification
    Brain-microglia
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 18 2012

    Question

    Hoping you'll be able to help me with something, I recently purchased an IBA1 antibody (ab15690). It is 100ug at a 3.1 mg/ml concentration. According to the datasheet this antibody does not need to be reconstituted but I would prefer to have a larger amount for ease of aliquoting and using etc.and also a conc. of 3.1mg/ml seems very high. Would it be ok to make the antibody up to 1ml (with 10mM PBS pH7.4) to achieve a dilution of 100ug/ml? Also, I wish to use this antibody for IHC-FrFl on rat brain tissue, would you be able to recommend the dilution factor I should use for this purpose if I am using the new concentration of antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 28 2013

    Answer

    This concentration is indeed quite high for an antibody so if you wish to dilute it in PBS then that is fine. I would say, that diluting it down to 1 mg/ml would be fine and is standard for most antibodies. Diluting it down to 100 ug/ml is quite a big dilution and I would not recommend this as it may affect the stability of this product especially when storing it at -20ºC or -80ºC.
    I notice that you are wanting to use this antibody for IHC-FrFl on rat brain tissue. As stated on the datasheet, although some customers have reported successful staining using this antibody on frozen sections, we do not routinely test this antibody on frozen sections, and we do not recommend it for use on these. For this reason, we have not suggested a dilution factor for this application. If you still want to proceed with this experiment I would suggest following the IHC-P guidelines so using 10-20 ug/ml of antibody per experiment.

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Nov 28 2013

    1-10 of 41 Abreviews or Q&A

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