Anti-Iba1 抗体 [1022-5] (ab15690)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)](https://www.abcam.co.jp/ps/products/15/ab15690/Images/ab15690-258713-anti-iba1-antibody-1022-5-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)")
Key features and details
- Mouse monoclonal [1022-5] to Iba1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-Iba1 antibody [1022-5]
Iba1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1022-5] to Iba1 -
由来種
Mouse -
特異性
Some customers have reported successful staining using this antibody on frozen sections, however ab15690 is not routinely tested on frozen sections and we do not recommend this antibody for use with frozen tissue.
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アプリケーション
適用あり: IHC-Pmore details
適用なし: IHC-Fr -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human Iba1.
Database link: P55008 -
ポジティブ･コントロール
- IHC-P: Human spleen. ICC/IF: Human induced microglia.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.05% KATHON™ CG/ICP
Constituents: 99% PBS, 0.5% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
1022-5 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15690の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (8) |
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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| 特記事項 |
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IHC-P
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
ターゲット情報
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機能
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. -
組織特異性
Detected in T-lymphocytes and peripheral blood mononuclear cells. -
配列類似性
Contains 2 EF-hand domains. -
翻訳後修飾
Phosphorylated on serine residues. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. - Information by UniProt
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参照データベース
- Entrez Gene: 199 Human
- Omim: 601833 Human
- SwissProt: P55008 Human
- Unigene: 76364 Human
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別名
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)
IHC image of Iba1 staining in human normal spleen formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15690, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)](https://www.abcam.co.jp/ps/products/15/ab15690/Images/ab15690-251915-anti-iba1-antibody-1022-5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)Immunohistochemical analysis of paraffin embedded human spleen sections, labelling Iba1 with ab15690.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)](https://www.abcam.co.jp/ps/products/15/ab15690/Images/ab15690-251916-anti-iba1-antibody-1022-5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [1022-5] (ab15690)Immunohistochemical analysis of paraffin embedded human spleen sections, labelling Iba1 with ab15690.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (113)
ab15690 は 113 報の論文で使用されています。
- Goebel U et al. Argon reduces microglial activation and inflammatory cytokine expression in retinal ischemia/reperfusion injury. Neural Regen Res 16:192-198 (2021). PubMed: 32788476
- Ding H et al. Elevated intracranial pressure induces IL-1ß and IL-18 overproduction via activation of the NLRP3 inflammasome in microglia of ischemic adult rats. Int J Mol Med 47:183-194 (2021). PubMed: 33236152
- Zheng Y et al. Electroacupuncture effects on the P2X4R pathway in microglia regulating the excitability of neurons in the substantia gelatinosa region of rats with spinal nerve ligation. Mol Med Rep 23:N/A (2021). PubMed: 33398365
- Lee DS & Kim JE Protein disulfide isomerase-mediated S-nitrosylation facilitates surface expression of P2X7 receptor following status epilepticus. J Neuroinflammation 18:14 (2021). PubMed: 33407649
- Wang YS et al. HCMV infection and IFITM3 rs12252 are associated with Rasmussen's encephalitis disease progression. Ann Clin Transl Neurol 8:558-570 (2021). PubMed: 33465303