製品の概要

  • 製品名

    Hydrogen Peroxide Blocking Reagent
  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details
  • 特記事項

    Hydrogen Peroxide Blocking Reagent ab64218 for use in IHC with detection based on HRP / peroxidase. 

    Note: The concentration of hydrogen peroxide in this product is 0.3%.


    IHC protocol suitable for use with Hydrogen Peroxide Blocking Reagent:
    For frozen sections, skip steps 1 and 2. 

    1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.

    2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.

    3. Add enough hydrogen peroxide blocking solution ab64218 to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.

    4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.

    5. Apply primary antibody in antibody diluent and incubate.

    6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.

    7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.

    8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC substrate and incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.

    9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.

    10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.


    Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

画像

  • Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block (ab64218) to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437. Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.

     

参考文献

This product has been referenced in:

  • Akca G  et al. The protective effect of astaxanthin against cisplatin-induced nephrotoxicity in rats. Biomed Pharmacother 100:575-582 (2018). Read more (PubMed: 29494988) »
  • Farrow N  et al. Epithelial disruption: a new paradigm enabling human airway stem cell transplantation. Stem Cell Res Ther 9:153 (2018). Read more (PubMed: 29895311) »
See all 7 Publications for this product

レビューと Q&A

1-4 of 4 Abreviews or Q&A

really good working kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We used this kit to reduce background staining produced by endogenous peroxidase .
Its works good and is easy to use, because of the dropper bottle.
We used the kit as a ready to use-kit and incubated the samples for 10minutes with the hydrogen peroxidase blocking reagent.

Abcam user community

Verified customer

投稿 Mar 12 2018

IHC-P with Ki67 antibody using H2O2 blocking reagant

Excellent Excellent 5/5 (Ease of Use)
Abreviews
H2O2 blocking reagant was used prior to protein blocking, and primary antibody was diluted 1:100 and incubated at RT for 1 hr. As the image indicates, the peroxidase activity was significantly blocked and there was minimal background staining.

Abcam user community

Verified customer

投稿 Aug 16 2016

Hydrogen peroxidase Blocking Solution

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Using HRP conjugated antibody may result in high, non-specific background staining. The non-specific background can be significantly reduced by pre-treatment of tissues with hydrogen peroxide prior to incubation with HRP conjugated antibody. Pictures show mouse gastrocnemius staining with no pre-treatment and pre-treatment with Hydrogen Peroxide Blocking solution from abcam. Results shows a significant improvement with an abolished background staining.

Dr. Francesco Elia Marino

Verified customer

投稿 Jul 23 2014

Question
Answer


ab64218 and ab94666 are exactly the same products but ab64218 is sold as 125 ml and ab94666 is sold as 60 ml.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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