Anti-HuR / ELAVL1 抗体 (ab28660)
Key features and details
- Rabbit polyclonal to HuR / ELAVL1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HuR / ELAVL1 antibody
HuR / ELAVL1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HuR / ELAVL1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chicken, Cow -
免疫原
Synthetic peptide corresponding to Human HuR/ ELAVL1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab28843) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28660の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Another band of approximately 52 kDa is also detected, we are unsure as the identity of this band.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Another band of approximately 52 kDa is also detected, we are unsure as the identity of this band. |
ターゲット情報
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機能
Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains. -
翻訳後修飾
Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1994 Human
- Entrez Gene: 15568 Mouse
- Omim: 603466 Human
- SwissProt: Q15717 Human
- SwissProt: P70372 Mouse
- Unigene: 184492 Human
- Unigene: 713744 Human
- Unigene: 119162 Mouse
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別名
- ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
- ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
- ELAV like 1 antibody
see all
画像
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All lanes : Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/ml
Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ELAVL1 knockout SW480 cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : HCT 116 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDaFalse colour image of Western blot: Anti-HuR / ELAVL1 antibody staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab28660 was shown to bind specifically to HuR / ELAVL1. A band was observed at 36 kDa in wild-type SW480 cell lysates with no signal observed at this size in ELAVL1 knockout cell line ab270485 (knockout cell lysate ab270508). To generate this image, wild-type and ELAVL1 knockout SW480 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab28660 は 5 報の論文で使用されています。
- Youn YH et al. Primary cilia control translation and the cell cycle in medulloblastoma. Genes Dev 36:737-751 (2022). PubMed: 35798383
- Liu Z et al. Circular RNA cIARS regulates ferroptosis in HCC cells through interacting with RNA binding protein ALKBH5. Cell Death Discov 6:72 (2020). PubMed: 32802409
- Green LC et al. Human antigen R as a therapeutic target in pathological cardiac hypertrophy. JCI Insight 4:N/A (2019). PubMed: 30668549
- Wang F et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res 27:416-439 (2017). PubMed: 28244490
- Workman E et al. U1A regulates 3' processing of the survival motor neuron mRNA. J Biol Chem 289:3703-12 (2014). PubMed: 24362020