Anti-HuR / ELAVL1 抗体 [EPR17397] - BSA and Azide free (ab242410)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17397] to HuR / ELAVL1 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, IP, WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HuR / ELAVL1 antibody [EPR17397] - BSA and Azide free
HuR / ELAVL1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17397] to HuR / ELAVL1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, IP, WB, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: SW480 and HAP1 cell lysates. IHC-P: Human cervix carcinoma, mouse cardiac muscle and rat cerebral cortex tissues. ICC/IF: HeLa cells. IP: HeLa whole cell lysate. Flow Cyt (intra): Jurkat (human acute T cell leukemia).
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特記事項
ab242410 is the carrier-free version of ab200342.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17397 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)
- Alexa Fluor® 647 Anti-HuR / ELAVL1 antibody [EPR17397] (ab209609)
- APC Anti-HuR / ELAVL1 antibody [EPR17397] (ab310841)
- PE Anti-HuR / ELAVL1 antibody [EPR17397] (ab310913)
- Alexa Fluor® 488 Anti-HuR / ELAVL1 antibody [EPR17397] (ab310967)
- Alexa Fluor® 594 Anti-HuR / ELAVL1 antibody [EPR17397] (ab311680)
- Alexa Fluor® 568 Anti-HuR / ELAVL1 antibody [EPR17397] (ab312955)
- Alexa Fluor® 555 Anti-HuR / ELAVL1 antibody [EPR17397] (ab313164)
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Compatible Secondaries
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Conjugation kits
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab242410の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains. -
翻訳後修飾
Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1994 Human
- Entrez Gene: 15568 Mouse
- Entrez Gene: 363854 Rat
- Omim: 603466 Human
- SwissProt: Q15717 Human
- SwissProt: P70372 Mouse
- Unigene: 184492 Human
- Unigene: 713744 Human
see all -
別名
- ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
- ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
- ELAV like 1 antibody
see all
画像
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All lanes : Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/5000 dilution
Lane 1 : Wild-type SW480 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : ELAVL1 knockout SW480 (Human colorectal adenocarcinoma cell line) cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab200342).
Lanes 1 - 2: Merged signal (red and green). Green - ab200342 observed at 36 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab200342 was shown to react with ELAVL1 in wild-type SW480 cells in western blot with loss of signal observed in ELAVL1 knockout sample. Wild-type and ELAVL1 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab200342 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling HuR / ELAVL1 with ab200342 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200342)
Control: PBS only.
Nuclear counter stain: DAPI. -
ab200342 staining HuR / ELAVL1 in Jurkat (human acute T cell leukemia) cells by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/23000. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200342)
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Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200342)
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200342)
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on rat cerebral cortex tissue tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200342)
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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HuR / ELAVL1 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200342 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200342 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab200342 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200342 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200342)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab242410 は 1 報の論文で使用されています。
- Simion V et al. A macrophage-specific lncRNA regulates apoptosis and atherosclerosis by tethering HuR in the nucleus. Nat Commun 11:6135 (2020). PubMed: 33262333