Human Vitronectin/S-Protein ELISA Kit (ab234577)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 19.9 pg/ml
- Range: 0.06 ng/ml - 4 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human Vitronectin/S-Protein ELISA Kit
Vitronectin/S-Protein キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Rec protein 6 3% -
サンプルの種類
Serum -
アッセイタイプ
Sandwich (quantitative) -
検出感度
19.9 pg/ml -
検出範囲
0.06 ng/ml - 4 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 114.9 107% - 119.2% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Vitronectin/S-Protein ELISA Kit (ab234577) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Vitronectin/S-Protein protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vitronectin/S-Protein with 19.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human Vitronectin/S-Protein Capture Antibody 1 x 600µl 10X Human Vitronectin/S-Protein Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Vitronectin/S-Protein Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
-
機能
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
組織特異性
Plasma. -
配列類似性
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
ドメイン
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
翻訳後修飾
Sulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
細胞内局在
Secreted, extracellular space. - Information by UniProt
-
別名
- Complement S Protein
- Epibolin
- S Protein
see all -
参照データベース
- Entrez Gene: 7448 Human
- Omim: 193190 Human
- SwissProt: P04004 Human
- Unigene: 2257 Human
関連製品
-
Alternative Versions
画像
-
The Vitronectin standard curve was prepared as described in Section 10.
-
The concentrations of Vitronectin were measured in duplicates, interpolated from the Vitronectin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitronectin concentration was determined to be 234.2 µg/mL in serum.
-
To learn more about the advantages of recombinant antibodies see here.
-
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab234577 は論文での使用が確認できていません。