Human VEGF Receptor 2 ELISA Kit (ab213476)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10 pg/ml
- Range: 117 pg/ml - 7500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human VEGF Receptor 2 ELISA Kit
VEGF Receptor 2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Human serum 8 2.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Human serum 3 5.8% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
10 pg/ml -
検出範囲
117 pg/ml - 7500 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 97 95% - 98% Serum 96 94% - 97% Hep Plasma 97 95% - 99% EDTA Plasma 95 89% - 98% Cit plasma 114 109% - 122% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human
非交差種: Cow -
製品の概要
Human VEGF Receptor 2 ELISA Kit (ab213476) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGF Receptor 2 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VEGF Receptor 2 with 10 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
VEGF R2 (vascular endothelial growth factor receptor 2) is 1356 amino acids long and is a class III receptor tyrosine kinase. VEGF R2 acts as a cell surface receptor for VEGFA, VEGFC, and VEGFD, where binding of these VEGFs to VEGF R2 activates several signaling cascades, including activation of PLCG1 (leading to the activation of protein kinase C), the MAP kinase signaling pathways, and the AKT1 signaling pathway. Furthermore, VEGF R2 plays an essential role in the regulation of angiogenesis, vascular development and permeability, embryonic hematopoiesis, and reorganization of the actin cytoskeleton. Finally, VEGF R2 is a major growth factor for endothelial cells and is the main facilitator of VEGF-induced endothelial proliferation, differentiation, and migration.
The standard is affinity purified.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human VEGF Receptor 2 Capture Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 20X Human VEGF Receptor 2 Detector Antibody 1 x 300µl Antibody Diluent 4BI 1 x 6ml Human VEGF Receptor 2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NBS 1 x 2ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
関連疾患
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
細胞内局在
Membrane. - Information by UniProt
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別名
- CD309
- CD309 antigen
- EC 2.7.10.1
see all -
参照データベース
- Entrez Gene: 3791 Human
- Omim: 191306 Human
- SwissProt: P35968 Human
- Unigene: 479756 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of VEGF Receptor 2 were measured in duplicate, interpolated from the VEGF Receptor 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%, plasma (citrate) 7.5%, and plasma (EDTA) 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 60 ng/mL in serum, 42 ng/mL in plasma (citrate) and 49 ng/mL in plasma (EDTA).
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The concentrations of VEGF Receptor 2 were measured in duplicate, interpolated from the VEGF Receptor 2 standard curve and corrected for sample dilution. Undiluted samples are as follows: plasma (heparin) 12.5% and HUVEC culture supernatant 80%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 44 ng/mL in plasma (heparin). Native signal of HUVEC culture supernatant at 80% was determined to be 0.4 ng/mL.
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Serum from ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 2.37 ng/mL with a range of 1.19 – 3.23 ng/mL.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab213476 は 3 報の論文で使用されています。
- Anwer KE et al. Design, Green Synthesis and Tailoring of Vitamin E TPGS Augmented Niosomal Nano-Carrier of Pyrazolopyrimidines as Potential Anti-Liver and Breast Cancer Agents with Accentuated Oral Bioavailability. Pharmaceuticals (Basel) 15:N/A (2022). PubMed: 35337128
- Salem SS et al. Synthesis of Silver Nanocomposite Based on Carboxymethyl Cellulose: Antibacterial, Antifungal and Anticancer Activities. Polymers (Basel) 14:N/A (2022). PubMed: 36015608
- Niepolski L et al. Circulating vascular endothelial growth factor receptor 2 levels and their association with lipid abnormalities in patients on hemodialysis. Biomed Rep 14:37 (2021). PubMed: 33692900