Human VEGF-165 ELISA Kit (ab273164)
Key features and details
- Sensitivity: 15.9 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human VEGF-165 ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Various 3.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Various 7.6% -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
15.9 pg/ml -
検出範囲
31.25 pg/ml - 1000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human VEGF165 ELISA kit (ab273164) is for the in-vitro qualitative and quantitative determination of VEGF165 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human VEGF165.
A capture antibody highly specific for VEGF-A has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of VEGF165 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed.
During the next incubation period the binding of the biotinylated anti-VEGF165 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of VEGF165 present in the samples and standards.
The absorbance of the colour complex is measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of VEGF165 in any sample tested.
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試験プラットフォーム
Microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests Biotinylated Antibody diluent 1 x 7ml Biotinylated Antibody VEGF-165 1 x 400µl HRP Diluent 1 x 12ml Plastic Plate Cover 2 units Standard diluent Buffer 1 x 15ml Stop Reagent 1 x 11ml Streptavidin-HRP 2 x 5µl TMB Substrate 1 x 11ml VEGF-165 Coated Microwell strips 1 unit VEGF-165 Standard 2 vials Wash Buffer 1 x 10ml -
研究分野
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細胞内局在
VEGF 165A: Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. -
参照データベース
- Entrez Gene: 7422 Human
- Omim: 192240 Human
- SwissProt: P15692 Human
- SwissProt: P15692-8 Human
- Unigene: 73793 Human
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab273164 は論文での使用が確認できていません。