Human TREM-1 ELISA Kit (ab270884)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.1 pg/ml
- Range: 31 pg/ml - 2000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human TREM-1 ELISA Kit
TREM1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 5.1% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 4 7.8% -
サンプルの種類
Saliva, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
7.1 pg/ml -
検出範囲
31 pg/ml - 2000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Saliva 110 106% - 110% Serum 100 97% - 101% Hep Plasma 95 95% - 96% EDTA Plasma 90 88% - 94% Cit plasma 92 86% - 96% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human TREM-1 ELISA kit (ab270884) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TREM-1 protein in human serum, plasmas, saliva, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human TREM-1 with 7.1 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
The triggering receptor expressed on myeloid cells (TREM), modulate the innate response either by amplifying or dampening Toll-Like Receptor (TLR) induced signals, and act to fine-tuning the inflammatory response. Trem-1 is a member of the TREM family of receptors encoded by a cluster of genes localized to the Human chromosome 6p21.1. The presence of TREM and TREM-like receptors are expressed on a variety of innate cells of the myeloid lineage including neutrophils, monocytes, macrophages, microglia, dendritic cells, among others. Soluble Trem-1 is thought to arise through the cleavage of the Trem-1 receptor by metalloproteinases, where it acts to negatively regulate TREM receptor signaling through neutralization of the respective ligands and minimize tissue damage due to prolonged inflammatory response.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human TREM-1 Capture Antibody 1 x 600µl 10X Human TREM-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human TREM-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. -
組織特異性
Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in normal cells than malignant cells. Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Strongly expressed in acute inflammatory lesions caused by bacteria and fungi. Isoform 2 was detected in the lung, liver and mature monocytes. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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別名
- CD354
- OTTMUSP00000018206
- TREM 1
see all -
参照データベース
- Entrez Gene: 54210 Human
- Omim: 605085 Human
- SwissProt: Q9NP99 Human
- Unigene: 283022 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human TREM-1 standard curve comparisonStandard Curve comparison between the original Human TREM-1 SimpleStep ELISA kit (ab185987) and current Human TREM-1 SimpleStep ELISA kit (ab270884).
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Human TREM-1 competitor curve comparisonStandard Curve comparison between Human TREM-1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human TREM-1 standard curve in Sample Diluent NS.The TREM-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TREM-1 in human saliva.The concentrations of TREM-1 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 820 pg/mL in saliva.
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Serum from eight individual healthy TREM-1 gender donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM-1 concentration was determined to be 180 pg/mL with a range of 80 – 269 pg/mL.
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Sandwich ELISA - Human TREM-1 ELISA Kit (ab270884)To learn more about the advantages of recombinant antibodies see here.
参考文献 (0)
ab270884 は論文での使用が確認できていません。
