製品の概要

  • 製品名

    Human Tissue Factor ELISA Kit
    Tissue Factor キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 8 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 6.9%
  • サンプルの種類

    Cell culture supernatant, Saliva, Urine, Cell culture extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    3.6 pg/ml
  • 検出範囲

    15.63 pg/ml - 1000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 110 105% - 116%
    Saliva 86 83% - 87%
    Urine 83 80% - 85%
    Cell culture extracts 105 100% - 107%
    Heparin Plasma 109 107% - 113%
    EDTA Plasma 115 111% - 118%
    Citrate Plasma 104 99% - 113%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat, Rabbit, Cow
  • 製品の概要

    Tissue factor in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor protein in plasma, saliva, cell culture supernatants, cell extracts, and urine.


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:



    • Samples in 1X Cell Extraction Buffer PTR: 7.6 pg/mL

    • Samples in Sample Diluent NS: 3.6 pg/mL

  • 特記事項

    Tissue factor (TF), also known as Coagulation factor III, F3 thromboplastin, and CD142 is a 263-amino acid single pass type I membrane protein encoded by the gene F3. TF is a 47 kDa single pass type I membrane protein consisting of an extracellular, transmembrane, and cytoplasmic domain. Tissue factor’s main role is in blood coagulation. The protein acts as a receptor for coagulation factor VII, which then forms the active complex FVIIa. This complex proteolytically activates downstream factors, including coagulation Factor IX and X, which leads to the formation of thrombin and fibrin clot formation.  Tissue Factor is in a class of proteins known as Cytokine Receptor class II family. This family is activated by cytokines, and play a role in angiogenesis and apoptosis.   

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab220653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Tissue factor were measured in duplicates, interpolated from the Tissue Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100%, plasma (heparin) 100%, plasma (EDTA) 100%, saliva 50% and A431 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 50 pg/mL in plasma (citrate), 78 pg/mL in plasma (heparin), 65.7 pg/mL in plasma (EDTA, 98 pg/mL in saliva) and 97.5 pg/mL in A431 cell culture supernatant.

  • The concentrations of Tissue Factor were measured in duplicates, interpolated from the Tissue Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: 50%, plasma (citrate) 50%, plasma (heparin) 50%, saliva 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 1,000 pg/mL in neat plasma (citrate), plasma (heparin), plasma (EDTA), saliva and 2,000 pg/mL in neat urine.

  • The concentrations of Tissue Factor were measured in duplicate and interpolated from the Tissue Factor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 10.4 ng/mL in U-87 cell extract and 100 ng/mL in A431 cell extract.

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参考文献

ab220653 has not yet been referenced specifically in any publications.

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