Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Human Soluble TNF Receptor II ELISA Kit (ab100643)

Submit a review Q&A (2)References (1)
Typical standard curve
  • Typical standard curve

Key features and details

  • Sensitivity: 5 pg/ml
  • Range: 2.74 pg/ml - 2000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human Soluble TNF Receptor II ELISA Kit
    Soluble TNF Receptor II キット 製品一覧

  • 検出方法

    Colorimetric

  • サンプルの種類

    Cell culture supernatant, Serum, Plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    < 5 pg/ml

  • 検出範囲

    2.74 pg/ml - 2000 pg/ml

  • 添加回収試験

    100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 102.65 91% - 112%
    Serum 104.92 95% - 115%
    Plasma 105.49 94% - 118%

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Abcam’s Soluble TNF Receptor II ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Soluble TNF Receptor II in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Soluble TNF Receptor II coated on a 96-well plate. Standards and samples are pipetted into the wells and Soluble TNF Receptor II present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Soluble TNF Receptor II antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Soluble TNF Receptor II bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Soluble TNF Receptor II 2 vials
    Recombinant Human Soluble TNF Receptor II Standard (lyophilized) 2 vials
    Soluble TNF Receptor II Microplate (12 x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • 研究分野

  • 関連性

    TNFRII (TNF Receptor II) is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF alpha but manifests a lower affinity to TNF beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNFRII consists of a 240 amino acid residue extracellular ligand binding portion, a 27 amino acid residue transmembrane segment and a 173 amino acid residue cytoplasmic domain. Soluble TNFRII (Soluble TNF Receptor II) is a 268 amino acid isoform containing the extracellular ligand-binding portion of TNFRII. It binds to TNF alpha and is capable of inhibiting TNF alpha induced activities by acting as a decoy receptor.

  • 別名

    • CD120b
    • Etanercept
    • p75
    • p80 TNF alpha receptor
    • TBP2
    • TBPII
    • TNFR2
    • TNFRII
    • Tumor necrosis factor binding protein 2
    • Tumor necrosis factor receptor 2
    • Tumor necrosis factor receptor superfamily member 1B
    • Tumor necrosis factor receptor superfamily member 1b, membrane form
    • Tumor necrosis factor receptor type II
    see all

  • 参照データベース

    画像

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100643

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100643

    参考文献 (1)

    ab100643 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab100643 は 1 報の論文で使用されています。

    • Nishikawa A  et al. Identification of definitive serum biomarkers associated with disease activity in primary Sjögren's syndrome. Arthritis Res Ther 18:106 (2016). PubMed: 27180164

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Hi,
    The ELISA that sounds suitable is ab100643 (etanarcept). Just to clarify- is this for drug levels? Is there an alternative version of the test for adalimumab/infliximab, as I couldn't find one.
    Kind Regards,

    Read More
    Answer

    Thank you for your recent message.

    I can confirm that this kit (Soluble human TNF Receptor II) is suitable for detecting Etanarcept which is an alternative name for TBPII. We have not specifically tested with these drugs. As long as your samples have detectable levels of TBPII and the samples are from cell culture supernatant, urine, serum, or from plasma this kit should work.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    Hi Technical,

    We have a customer who is interested in the following kits;

    IL-6 (ab46044)

    CRP (ab108826)

    TNFR2 (ab100643)

    TNFR1 (ab100642)

    The customer’s correspondence;

    I was wondering if you could help me – We’re coming up on the time when we’ll actually order these kits and we want to make sure that we’ve got everything lined up so that testing goes smoothly.

    I was wondering if you or a colleague could provide the following information for the kits listed above:

    1. Type of vacutainer tube needed for each marker

    2. Approximate amount of whole blood needed per patient, per marker

    3. Time limit for the storage of samples for each marker (i.e. 1 month? 1 year?)

    As I am not quite sure when to locate this information, so if you could help me out here, I would really appreciated it.

    Hoping to hear back from you soon.

    Kind regards,

    Read More
    Answer

    Thank you for your enquiry regarding these kits.

    I would suggest reading through the Protocol Booklets for each of these kits provided on the product datasheets. These documents contain specific information about the sample types, sample preparations, protocol, instruments and equipment required for the assays. Whole blood samples are not used to test these kits mainly serum or plasma instead.

    ab46044: https://www.abcam.com/high-sensitivity-il6-human-elisa-kit-2-x-96-well-plates-ab46044.html

    ab100642: https://www.abcam.com/ps/products/100/ab100642/documents/ab100642-Soluble-TNF-Receptor-I-Human-ELISA-Kit-ab100642-protocol-plain.pdf

    ab100643: https://www.abcam.com/ps/products/100/ab100643/documents/ab100643-Soluble-TNF-Receptor-II-Human-ELISA-Kit-ab100643-protocol-plain.pdf

    ab108826: https://www.abcam.com/ps/products/108/ab108826/documents/ab108826%20C%20Reactive%20Protein%20Human%20ELISA_v2%20(Website).pdf

    As you are aware our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. Therefore please advsie yiur customer to use teh product as soon as possible after purchase to make sure that we can provide the best technical support within the guarantee period.

    The specific storage instructions can also be found on the product datasheets. It is not clear if the customer would like to use these products in serum from normal, or drug treated or diseased (pathological conditions) individuals. We would suggest optimizing the assay and using standards.

    Let me know if you haveany further questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com