Human SOCS3 peptide (ab16199)
Key features and details
- Purity: > 90% HPLC
- Suitable for: WB, Blocking
製品の詳細
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製品名
Human SOCS3 peptide
SOCS3 タンパク質・ペプチド 製品一覧 -
精製度
> 90 % HPLC. -
Animal free
No -
由来
Synthetic -
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生物種
Human
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関連製品
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Corresponding Antibody
特性
Our Abpromise guarantee covers the use of ab16199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Blocking - Blocking peptide for Anti-SOCS3 antibody (ab16030)
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製品の状態
Lyophilized -
備考
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
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再構成Reconstitute with 100 ul of deionised water (or equivalent).
関連情報
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別名
- ATOD4
- CIS 3
- CIS-3
see all -
機能
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. -
組織特異性
Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients. -
配列類似性
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
ドメイン
The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. -
翻訳後修飾
Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1. - Information by UniProt
画像
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Western blot - Human SOCS3 peptide (ab16199)The 30kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SOCS3.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab16199 は論文での使用が確認できていません。
