Human SIRT2 ELISA Kit (ab227895)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human SIRT2 ELISA Kit
SIRT2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5 2.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 3 4.1% -
サンプルの種類
Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
3 pg/ml -
検出範囲
15.6 pg/ml - 1000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 95 93% - 96% Cell culture extracts 104 93% - 116% Tissue Extracts 108 94% - 117% Hep Plasma 84 82% - 86% EDTA Plasma 84 83% - 85% Cit plasma 83 80% - 84% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human SIRT2 ELISA Kit (ab227895) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SIRT2 protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SIRT2 with 3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human SIRT2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human SIRT2 Capture Antibody (Lyophilized) 1 vial Human SIRT2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
組織特異性
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
配列類似性
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
発生段階
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
翻訳後修飾
Phosphorylated at the G2/M transition of the cell cycle. -
細胞内局在
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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別名
- FLJ35621
- FLJ37491
- NAD dependent deacetylase sirtuin 2
see all -
参照データベース
- Entrez Gene: 22933 Human
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- Unigene: 466693 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native SIRT2 based on a 150 µg/ml extract load in Human Fetal Brain Homogenate Extract, 600 µg/ml in U-87 MG Extract, 250 µg/ml in PC-3 Extract, and 125 µg/ml in THP-1 Extract. The concentrations of SIRT2 were measured in duplicate and interpolated from the SIRT2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SIRT2 concentration was determined to be 998 pg/mL in Human Fetal Brain Homogenate Extract, 862 pg/mL in U-87 MG Extract, 978 pg/ml in PC-3 Extract, and 925 pg/ml in THP-1 Extract.
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The concentrations of SIRT2 were measured in duplicates, interpolated from the SIRT2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab227895 は論文での使用が確認できていません。