製品の概要

  • 製品名
    Anti-Human Serum Albumin antibody
    Human Serum Albumin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Human Serum Albumin
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: Sandwich ELISA, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified human albumin.

  • ポジティブ･コントロール
    • IHC-P: Human liver tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [15C7] to Human Serum Albumin (ab10241). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [15C7] to Human Serum Albumin (ab10241) as Capture.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
This antibody may be diluted to a titer of 1/50-1/1000 in an ABC method. We suggest an incubation period of 30 minutes at room temperature.

ターゲット情報

  • 機能
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 組織特異性
    Plasma.
  • 関連疾患
    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • 配列類似性
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻訳後修飾
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
    • 別名
      • alb antibody
      • ALBU_HUMAN antibody
      • Albumin (32 AA) antibody
      • Albumin (AA 34) antibody
      • Albumin antibody
      • Analbuminemia antibody
      • Bisalbuminemia antibody
      • Cell growth inhibiting protein 42 antibody
      • DKFZp779N1935 antibody
      • Dysalbuminemic hyperthyroxinemia antibody
      • Growth inhibiting protein 20 antibody
      • HSA antibody
      • Hyperthyroxinemia dysalbuminemic antibody
      • PRO0883 antibody
      • PRO0903 antibody
      • PRO1341 antibody
      • Serum albumin antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (ab2406)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (ab2406)

      Formalin-fixed, paraffin-embedded human liver tissue stained for Human Serum Albumin using ab2406 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (ab2406)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (ab2406)
      Ab2406 staining human normal liver. Staining is localised to the cytoplasm and secreted.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be re
    • Sandwich ELISA - Anti-Human Serum Albumin antibody (ab2406)
      Sandwich ELISA - Anti-Human Serum Albumin antibody (ab2406)
      Standard Curve for Human Serum Albumin (Analyte: Human Serum Albumin protein (ab67670)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [15C7] to Human Serum Albumin (ab10241) at 1µg/ml and Detector Antibody Rabbit polyclonal to Human Serum Albumin (ab2406) at 0.5µg/ml.

    参考文献

    This product has been referenced in:
    • Zheng Z  et al. Hepatitis C virus mediated chronic inflammation and tumorigenesis in the humanised immune system and liver mouse model. PLoS One 12:e0184127 (2017). Read more (PubMed: 28886065) »
    • Lichtmannegger J  et al. Methanobactin reverses acute liver failure in a rat model of Wilson disease. J Clin Invest 126:2721-35 (2016). Read more (PubMed: 27322060) »

    See all 12 Publications for this product

    ab2406 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    8 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-Human Serum Albumin antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (10% Gel)
    Loading amount
    30 µg
    Specification
    skeletal muscle
    Blocking step
    Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
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    Abcam user community

    Verified customer

    投稿 Jun 13 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Human Serum Albumin antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skeletal muscle)
    Permeabilization
    No
    Specification
    skeletal muscle
    Fixative
    none
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 May 28 2018

    Hallo,

    I´ve just three questions about this product:

    Do you know the antigen that was used for immunization of the rabbit (full length HSA or just a part of the protein??)

    Do you know if there is a cross reactivit...

    Read More

    Thank you for your enquiry and your interest.

    I can confirm that the immunogen used to raise this antibody was a full length purified human albumin. We do not have any cross-reactivity data with other species but a soon as we have further inf...

    Read More

    what should be used for blocking for sELISA?
    Would BSA OK to ue or would Abs crossreact?

    Thank you for contacting us.

    I have heard back from the lab:
    For ab2406, for use with human tissues, BSA may work but for other species it may crossreact.
    For ab10241, it is not crossreacting with BSA.

    Thus using BSA would be ...

    Read More

    I may want to test ab10241 in IHC-P.

    Thank you very much for your interest in ab10241.

    To our knowledge, ab10241 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab10241 now, test it in IHC-P and submit feedback to us in the form ...

    Read More

    Hello,

    We would like to remove albumin from lipoprotein contained in 100 ul of plasma that contain ˜4 mg of albumin using your polyclonal antibody ab2406 (the idea is to add excess antibody). Although it says that it has not been validated f...

    Read More

    Thank you for your enquiry regarding ab2406.

    The protein concentration for this antibody is 3.3 g/L. I regret to inform you that we do not have any information that explains how many mg of albumin can be bound per ug of antibody.

    Si...

    Read More

    Bonjour, ma question initiale portait sur trois anticorps dont j'avais donné le numéro d'article (désolée je suis au labo et n'ai que mon IPhone donc pas de détails supplémentaires a vous fournir) qui étaient tous fait contre la protéine native et je d...

    Read More

    Ces trois anticorps que vous avez cité dans votre émail - ab31657, ab2406 et ab34856 - ne sont pas testés en Western blot. Néanmoins, si vous souhaitez tester un de ces trois anticorps, je vous conseillerais le ab2406 car il est au moins testé aussi da...

    Read More

    I would like to test ab2406 for use in WB

    DISCOUNT CODE: *** Expiration date: April 5th, 2012 I am very pleased to hear you would like to accept our offer and test ab2406 in WB. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an A...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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