Human Semaphorin 7A ELISA Kit (ab272193)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24.7 pg/ml
- Range: 70.31 pg/ml - 4500 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Semaphorin 7A ELISA Kit
Semaphorin 7a キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 2.52% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 6.62% -
サンプルの種類
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
24.7 pg/ml -
検出範囲
70.31 pg/ml - 4500 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 107 104% - 112% Hep Plasma 103 101% - 105% EDTA Plasma 104 103% - 105% Cit plasma 117 116% - 119% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Semaphorin 7A ELISA kit (ab272193) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Semaphorin 7A protein in serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Semaphorin 7A with 24.7 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Semaphorins constitute a large class of soluble and membrane bound proteins that are known to be involved in critical immune responses and serve neuronal functions. Semaphorin 7A, also known as CD108, is a glycosylphosphatidylinositol-anchored semaphorin that promotes axon outgrowth through beta1-integrin receptors and contributes to the formation of the lateral olfactory tract. Promotes attachment, spreading and dendrite outgrowth in melanocytes and plays an important role in modulating inflammation and T-cell mediated immune response. Mouse, rat. cow and monkey share 89, 89%, 86% and 99% sequence homology, respectively.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human Semaphorin 7A Capture Antibody 1 x 600µl 10X Human Semaphorin 7A Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Semaphorin 7A Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. -
組織特異性
Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney. -
配列類似性
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
細胞内局在
Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes. - Information by UniProt
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別名
- CD 108
- CD108
- CD108 antigen
see all -
参照データベース
- Entrez Gene: 8482 Human
- Omim: 607961 Human
- SwissProt: O75326 Human
- Unigene: 24640 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The Semaphorin 7A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Semaphorin 7A were measured in duplicate, interpolated from the Semaphorin 7A standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Semaphorin 7A concentration was determined to be 14,101 pg/mL in serum, 11,909 pg/mL in plasma (citrate) and 14,381 pg/mL in plasma (EDTA), and 10,604 pg/ml in plasma (heparin).
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272193 は論文での使用が確認できていません。