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Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897)

Submit a review Q&A (1)References (5)
Typical Standard Curve

    Key features and details

    • Sensitivity: 7.4 ng/ml
    • Range: 6.25 ng/ml - 400 ng/ml
    • Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    製品の概要

    • 製品名

      Human Retinol binding protein 4 ELISA Kit (RBP4)
      RBP4 キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Overall 5.2%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Overall 10%

    • サンプルの種類

      Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      = 7.4 ng/ml

    • 検出範囲

      6.25 ng/ml - 400 ng/ml

    • 添加回収試験

      97 %

    • 全工程の試験時間

      4h 00m

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Abcam’s Retinol binding protein 4 (RBP4) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Retinol binding protein 4 concentrations in plasma, serum, saliva, urine, milk, cerebrospinal fluid and cell culture supernatants.


      A Retinol binding protein 4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Retinol binding protein 4specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Retinol binding protein 4 captured in plate.


      Get higher sensitivity in only 90 minutes with Human Retinol Binding Protein 4 ELISA Kit (ab196264) from our SimpleStep ELISA® range.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • 試験プラットフォーム

      Microplate

    製品の特性

    画像

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve Using ab108897.

    参考文献 (5)

    ab108897 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108897 は 5 報の論文で使用されています。

    • Torabi R & Ghourchian H Ultrasensitive nano-aptasensor for monitoring retinol binding protein 4 as a biomarker for diabetes prognosis at early stages. Sci Rep 10:594 (2020). PubMed: 31953481
    • Tayebi SM  et al. Plasma retinol-binding protein-4 and tumor necrosis factor-a are reduced in postmenopausal women after combination of different intensities of circuit resistance training and Zataria supplementation. Sport Sci Health 15:551-558 (2019). PubMed: 31885723
    • Al-Attar A  et al. Data correlations between gender, cytomegalovirus infection and T cells, NK cells, and soluble immune mediators in elderly humans. Data Brief 8:536-44 (2016). PubMed: 27508213
    • Gonzalez-Calero L  et al. Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis? Sci Rep 6:19667 (2016). PubMed: 26792617
    • Pirazzi C  et al. PNPLA3 has retinyl-palmitate lipase activity in human hepatic stellate cells. Hum Mol Genet N/A:N/A (2014). PubMed: 24670599

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    What are the immunogens for the antibodies in these kits?

    Read More
    Answer

    Thank you for your patience with this reply.

    I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

    1) Apolipoprotein A1 Human ELISA Kit
    2) Apolipoprotein E Human ELISA Kit
    3) Haptoglobin Human ELISA Kit
    4) Hemopexin Human ELISA Kit
    5) Kininogen Human ELISA Kit
    6) Retinol binding Protein 4 Human ELISA Kit

    I'm sorry that this information could not be more helpful. Please contact us with any other questions.

    Read More

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