Human Anti-Respiratory syncytial virus IgG ELISA Kit (RSV) (ab108765)

Submit an Abreview Q&A (4)Specific References (1)

製品の概要

  • 製品名
    Human Anti-Respiratory syncytial virus IgG ELISA Kit (RSV)
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Pos.Serum 24 8.5%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Pos.Serum 12 6%
  • サンプルの種類
    Serum, Heparin Plasma, Citrate Plasma
  • アッセイタイプ
    Indirect
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s anti-Respiratory syncytial virus (RSV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Respiratory syncytial virus in Human serum and plasma.

     

    A 96-well plate has been precoated with Respiratory syncytial virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Respiratory syncytial virus -specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Respiratory syncytial virus IgG sample captured in plate.

  • アプリケーション
    適用あり: Indirect ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Indirect ELISA Use at an assay dependent concentration.

プロトコール

参考文献

This product has been referenced in:
  • Grandin C  et al. Evidence for an intranasal immune response to human respiratory syncytial virus infection in cynomolgus macaques. J Gen Virol 96:782-92 (2015). Read more (PubMed: 25537374) »

See 1 Publication for this product

ab108765 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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4 reviews or Q&A

10
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-Which is the origin of your positive sera?
-Is it human sera from positive volunteers?
-Is it possible to buy only the positive control sera?

The controls in the RSV IgG ELISA are human sera but are not available for sale apart from the kit.

Can you tell me if the coated antigens should recognize antibodies generated against the A2 strain for RSV?

The antigens are from the Long strain. The kit has only been tested with clinical samples in which the virus strain that initiated the antibody production is unknown. The samples are expected to contain a mixture of antibodies against different RSV str...

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Dear Abcam representative,



Does the Respiratory Syncytial Virus (RSV) IgG Human ELISA Kit (cat. no. ab108765) cross-react with nonhuman primate – African green monkey?


Thank you for your patience in awaiting our reply. We have spoken with the laboratory and they are unsure whether this kit will react with African Green Monkey.

Phone call requesting information on the epitope recognised by the Respiratory Syncytial Virus (RSV) IgG Human ELISA Kit (ab108765)

Thank you for contacting us and your interest in the Respiratory Syncytial Virus (RSV) IgG Human ELISA Kit (ab108765). I am sorry for the delay in getting back to you.

The kit is intended to be used to quantify the human anti-RSV IgG present i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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