製品の概要

  • 製品名

    Human Protein S ELISA Kit
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    EDTA Plasma 5 1.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    EDTA Plasma 3 2.5%
  • サンプルの種類

    Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    0.4 ng/ml
  • 検出範囲

    3.12 ng/ml - 200 ng/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 88.7 86% - 93.4%
    Heparin Plasma 85.7 78.6% - 91.1%
    EDTA Plasma 89.3 86.6% - 92.8%
    Citrate Plasma 90.4 87% - 94%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Pig
  • 製品の概要

    Protein S in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Protein S protein in human serum and plasma samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 特記事項

    Protein S (vitamin K-dependent protein S) is an anticoagulant plasma protein. Protein S is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis. Protein S deficiency causes autosomal dominant (THP5) [MIM: 612336] and autosomal recessive (THP6) [MIM: 614514] thrombophilia. The autosomal dominant thrombophilia due to Protein S deficiency (THPH5) is hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. Based on the plasma levels of total and free Protein S as well as the serine protease-activated protein C cofactor activity, three types of THPH5 have been described: type I, characterized by reduced total and free Protein S levels together with reduced anticoagulant activity; type III, in which only free Protein S antigen and Protein S activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free Protein S antigen, but low cofactor activity. The autosomal recessive thrombophilia due to Protein S deficiency (THPH6) is a very rare and severe hematologic disorder resulting in thrombosis and secondary hemorrhage usually beginning in early infancy. Some affected individuals develop neonatal purpura fulminans, multifocal thrombosis, or intracranial hemorrhage.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Human Protein S Capture Antibody 1 x 600µl
    10X Human Protein S Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human Protein S Lyophilized Purified Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究分野

  • 関連性

    Function: Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
  • 別名

    • PROS
    • PROS1
    • Protein S (alpha)
    • Protein Sa
    • PS21
    • PS22
    • PS23
    • PS24
    • PS25
    • PSA
    • THPH5
    • THPH6
    • Vitamin K dependent plasma protein S
    • Vitamin K dependent protein S
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab190808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • 200 X diluted serum samples from 10 apparently healthy male donors were measured in triplicates using this kit. Interpolated data values corrected for sample dilution are graphed in ng of Protein S per mL of serum (mean +/- SD, n=3). The mean of Protein S concentration of these serum samples was determined to be 22,623 pg/mL with a range of 19,138 – 28,150 ng/mL.

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参考文献

ab190808 has not yet been referenced specifically in any publications.

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