製品の概要

  • 製品名

    Human Protein C ELISA Kit
    Protein C キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall = 4.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall = 7.3%
  • サンプルの種類

    Cell culture supernatant, Saliva, Milk, Urine
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    = 3 ng/ml
  • 検出範囲

    3.13 ng/ml - 200 ng/ml
  • 添加回収試験

    = 98 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Saliva 94% - 99%
    Milk 94% - 98%
    Urine 89% - 93%

  • 全工程の試験時間

    4h 0m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Human
  • 製品の概要

    Abcam’s Protein C Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Protein C in urine, saliva, milk, and cell culture supernatant.


    A Protein C specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Protein C specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Protein C captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Protein C Antibody (Lyophilized) 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Protein C Microplate (12 x 8 well strips) 1 unit
    Protein C Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究分野

  • 機能

    Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids.
  • 組織特異性

    Plasma; synthesized in the liver.
  • 関連疾患

    Defects in PROC are the cause of thrombophilia due to protein C deficiency, autosomal dominant (THPH3) [MIM:176860]. A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Individuals with decreased amounts of protein C are classically referred to as having type I protein C deficiency and those with normal amounts of a functionally defective protein as having type II deficiency.
    Defects in PROC are the cause of thrombophilia due to protein C deficiency, autosomal recessive (THPH4) [MIM:612304]. A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. It results in a thrombotic condition that can manifest as a severe neonatal disorder or as a milder disorder with late-onset thrombophilia. The severe form leads to neonatal death through massive neonatal venous thrombosis. Often associated with ecchymotic skin lesions which can turn necrotic called purpura fulminans, this disorder is very rare.
  • 配列類似性

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾

    The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
    N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
  • Information by UniProt
  • 別名

    • Activation peptide
    • Anticoagulant protein C
    • Autoprothrombin IIA
    • Blood coagulation factor XIV
    • proC
    • PROC_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab137987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab137987

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参考文献

ab137987 has not yet been referenced specifically in any publications.

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