製品の概要

  • 製品名

    Human Prekallikrein and Kallikrein ELISA Kit
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall = 4.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall = 8.9%
  • サンプルの種類

    Cell culture supernatant, Saliva, Milk, Serum, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    > 1.2 ng/ml
  • 検出範囲

    1.25 ng/ml - 80 ng/ml
  • 添加回収試験

    = 98 %

  • 全工程の試験時間

    4h 00m
  • 種交差性

    交差種: Human
  • 製品の概要

    Abcam’s Prekallikrein and Kallikrein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prekallikrein and Kallikrein levels in plasma, serum, saliva, milk, cerebrospinal fluid and cell culture supernatants.


    A Prekallikrein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Prekallikrein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Prekallikrein and Kallikrein captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab171015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Prekallikrein measured in various biologicals showing mass (ng) per mL of sample tested.

  • Representative Standard Curve using ab171015

プロトコール

参考文献

This product has been referenced in:

  • Harbaum L  et al. Reduced plasma levels of small HDL particles transporting fibrinolytic proteins in pulmonary arterial hypertension. Thorax 74:380-389 (2019). Read more (PubMed: 30478197) »
See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab171015.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録