Human PDGF AA ELISA Kit (ab100622)
Key features and details
- Sensitivity: 40 pg/ml
- Range: 40 pg/ml - 30000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human PDGF AA ELISA Kit
PDGF AA キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 40 pg/ml -
検出範囲
40 pg/ml - 30000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 92.54 82% - 102% Serum 76.71 70% - 83% Plasma 78.06 72% - 85% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s PDGF AA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AA in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PDGF AA coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-Human PDGF AA 2 vials PDGF AA Microplate (12 x 8 wells) 1 unit Recombinant Human PDGF AA Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
ドメイン
The long form contains a basic insert which acts as a cell retention signal. -
細胞内局在
Secreted. - Information by UniProt
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別名
- PDGF A
- PDGF A chain
- PDGF subunit A
see all -
参照データベース
- Entrez Gene: 5154 Human
- Omim: 173430 Human
- SwissProt: P04085 Human
- Unigene: 535898 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab100622 は 3 報の論文で使用されています。
- Chambliss C et al. Elevated neuregulin-1β levels correlate with plasma biomarkers of cerebral injury and high stroke risk in children with sickle cell anemia. Endocr Metab Sci 3:N/A (2021). PubMed: 35935682
- Dai Q et al. Modulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles. J Inflamm Res 12:335-343 (2019). PubMed: 31908518
- Wood MD et al. Neurofibromin knockdown in glioma cell lines is associated with changes in cytokine and chemokine secretion in vitro. Sci Rep 8:5805 (2018). PubMed: 29643433