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Anti-Human Papillomavirus 16 (E7) 抗体 [TVG 701Y] (ab20191)

Submit a review Q&A (5)References (6)

Key features and details

  • Mouse monoclonal [TVG 701Y] to Human Papillomavirus 16 (E7)
  • Reacts with: Human papillomavirus
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Human Papillomavirus 16 (E7) antibody [TVG 701Y]

  • 製品の詳細

    Mouse monoclonal [TVG 701Y] to Human Papillomavirus 16 (E7)

  • 由来種

    Mouse

  • 種交差性

    交差種: Human papillomavirus

  • 免疫原

    Full length protein (HPV 16 E7) synthesised from S. pombe.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A/G purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    TVG 701Y

  • ミエローマ

    NS0/1

  • アイソタイプ

    IgG2a

  • 研究分野

ターゲット情報

  • 関連性

    E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription

  • 細胞内局在

    Nuclear

  • 参照データベース

    • 別名

      • E7 antibody
      • HPV16 E7 antibody
      • Protein E7 antibody
      • Transforming protein antibody

    参考文献 (6)

    ab20191 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab20191 は 6 報の論文で使用されています。

    • Chen Y  et al. In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system. Antiviral Res 178:104794 (2020). PubMed: 32298665
    • Kitamura K  et al. Evaluation of HPV16 E7 expression in head and neck carcinoma cell lines and clinical specimens. Sci Rep 10:22138 (2020). PubMed: 33335126
    • von Witzleben A  et al. Correlation of HPV16 Gene Status and Gene Expression With Antibody Seropositivity and TIL Status in OPSCC. Front Oncol 10:591063 (2020). PubMed: 33575210
    • Kannan A  et al. Genetic Mutation and Exosome Signature of Human Papilloma Virus Associated Oropharyngeal Cancer. Sci Rep 7:46102 (2017). PubMed: 28383029
    • Harris M  et al. Combined treatment of the experimental human papilloma virus-16-positive cervical and head and neck cancers with cisplatin and radioimmunotherapy targeting viral E6 oncoprotein. Br J Cancer 108:859-65 (2013). PubMed: 23385729
    • Tommasino M  et al. HPV16 E7 protein associates with the protein kinase p33CDK2 and cyclin A. Oncogene 8:195-202 (1993). PubMed: 8380917

    レビューと Q&A

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    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Question

    How was this HPV16 antibody validated for ELISA?

    Read More
    Answer

    Thank you for contacting us and for your patience.

    The supplying lab let us know the following:

    We do not have a protocol sincewe are provided our antibodies by outside scientists and we tend to see only the information that is provided to us.



    I am very sorry to not be able to help you better.
    Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Hellhttps://www.abcam.com/ab30731o
    I recently ordered HPV 18 E7 antibody..it seemed working very well. I want to get 16 E7 antibody as well. You have different cat numbers for the same antibody. Which one do you recommend, I will be mainly using it WB/IF from CaSki/SiHa cells.
    (I found these products:
    Anti-Human Papillomavirus 16 (E7) antibody [TVG 701Y] (ab20191)
    Anti-Human Papillomavirus 16 (E7) antibody [289-17013] (ab30731))
    Thank you

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.

    These two monoclonal antibodies recognize the same protein (Human Papillomavirus 16 (E7)) but are from different clones.

    https://www.abcam.com/ab20191:

    https://www.abcam.com/ab30731:

    As you can see on the datasheets, the concentration, the tested application and the storage buffer are different.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    Dear technical team,

    1. Our customer would like to know detail immunogen information.
    Her samplehas mutation on 22˜26 a.a. and needsantibody does not detect 22-26 a.a.

    2.Let me know recent lot# to check the concentration.

    Best regards,

    Read More
    Answer

    Thank you for your enquiry. I am pleased to help answer your questions.

    1. I have contacted the originator and regrettably they are not able to provide any further information on the immunogen on this occasion.We aim to provide as much information as possible to customers and I am sorry I am unable to give you more details on this occasion.

    2.The current lots we have in stock are as follows:

    GR62142 is 1.3 mg/ml
    GR74578 is 1.35 mg/ml

    I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Question

    Would like some literature references for this antibody clone.

    Read More
    Answer

    Thank you for your call today and for your questions about ab20191.

    I have done a search on google scholar for the clone TVG 701Y, and there are several publications listed:

    http://scholar.google.com/scholar?q=TVG+701Y&hl=en&as_sdt=1%2C22&as_sdtp=on

    I have downloaded the pdf files for the first two listings and attached them to this email.

    I hope this information will be helpful in determining whether this antibody will work with your studies. If there is anything else that we can do for you, please don't hesitate to ask. Have a great weekend!

    Read More

    Question

    To whom it may concern, I'm interested in using a HPV 16 E7 monoclonal antibody targeting the C terminus of the protein, for flow and/or western blot application. Can you tell me the epitope that your Human Papillomavirus 16 (E7) antibody [TVG701Y] binds to, and if you have what I'm looking for?

    Read More
    Answer

    Thank you for contacting Abcam.   I have contacted the lab about ab20191, which is a Human Papillomavirus 16 (E7) antibody [TVG701Y] and can be used in flow cytometry and western blot. However the lab has told me that we do not know what the immunogen sequence is for this antibody.   This paper might be of help to you though:   http://www.ncbi.nlm.nih.gov/pubmed?term=8380917    If there is anything else I can help you with, please let me know.  

    Read More

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