製品の概要

  • 製品名

    Human PAI1+tPA ELISA Kit
    PAI1 + tPA キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5 6.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 14.7%
  • サンプルの種類

    Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    16 pg/ml
  • 検出範囲

    39 pg/ml - 2500 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Urine 88 82% - 91%
    Serum 89 80% - 100%
    Cell culture media 99 92% - 108%
    Hep Plasma 103 93% - 111%
    EDTA Plasma 67 66% - 70%
    Cit plasma 82 80% - 84%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Human
  • 製品の概要

    PAI1 (SERPINE1) - Tissue Plasminogen Activator (TPA) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 (SERPINE1) - Tissue Plasminogen Activator (TPA) protein in serum, plasma, cell culture supernatant, and urine.


     


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 特記事項

    Tissue plasminogen activator (tPA or PLAT) is a serine protease that catalyzes the conversion of plasminogen to plasmin, which is integral to blood clot breakdown. Plasminogen activator inhibitor-1 (PAI-1 or Serpin E1) is a serine protease inhibitor that blocks the function of tPA. PAI-1 and tPA form a complex that is stable and non-covalent. The PAI-1/tPA complex appears to be predictive of risk for myocardial infarction. This assay uses a PAI-1 specific capture antibody and a tPA-specific detector antibody to specifically detect PAI1/tPA complexes in Human biological samples. The reference protein is a non-covalent complex of PAI 1 and tPA.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab192151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of PAI1-TPA complex were measured in duplicate and interpolated from the PAI1-TPA complex standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1-TPA complex concentration was determined to be 2,040 pg/mL in serum and 2,160 pg/mL in plasma (heparin).

  • Serum from 10 apparently healthy male donors was measured in duplicate. The mean PAI1-TPA complex concentration was determined to be 4,126 pg/mL with a range of 2,764- 5,584 pg/mL in male donors.

  • Recombinant proteins for PAI1, TPA, and PAI1-TPA complex were tested in PAI1 SimpleStep ELISA, TPA SimpleStep ELISA and PAI1-Complex SimpleStep ELISA to determine specificity of the PAI-TPA complex ELISA. Background-subtracted data values are graphed. The PAI1-TPA Complex assay does not detect free PAI1 or TPA protein.

  • PAI1-TPA complex, PAI1 protein and TPA protein were measured in 8-fold diluted serum and plasma. PAI1, TPA, and PAI1-TPA complex values were measured and interpolated from their respective Standard Curve diluted in Sample Diluent NS or Sample Diluent 25BP. Mean +/-SD, n=2, are graphed.

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参考文献

ab192151 has not yet been referenced specifically in any publications.

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