製品の概要

  • 製品名

    Human PAI1 ELISA Kit (SERPINE1)
    PAI1 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    HeLa extract 5 4.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    HeLa extract 3 9.1%
  • サンプルの種類

    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    21 pg/ml
  • 検出範囲

    93.75 pg/ml - 6000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 113 109% - 118%
    Plasma 115 115% - 116%
    Cell culture media 104 91% - 111%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Human
  • 製品の概要

    PAI1 (SERPINE1) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI in human serum, plasma, cell culture supernatants and cell extracts.


    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


     Sensitivity:


    Samples diluted in Sample Diluent 25BP - 48 pg/mL


    Samples diluted in 1X Cell Extraction Buffer PTR - 21 pg/mL

  • 特記事項

    Plasminogen activator inhibitor-1 (PAI-1) is encoded by the SERPINE1 gene. The PAI-1 protein is a serine protease inhibitor (serpin) that functions as the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA). The inhibition of tPA and uPA leads to increased occurrence and persistence of blood clots to form.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Human PAI1 Capture Antibody 1 x 600µl
    10X Human PAI1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human PAI1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究分野

  • 機能

    Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
  • 組織特異性

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 関連疾患

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 配列類似性

    Belongs to the serpin family.
  • 翻訳後修飾

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • Endothelial plasminogen activator inhibitor
    • PAI
    • PAI-1
    • PAI1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpin E1
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • SERPINE1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab184863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of PAI1 standard curve using Sample Diluent 25BP. Background-subtracted data values (mean +/- SD) are graphed.

  • Example of PAI1 standard curve using 1X Cell Extraction Buffer PTR as a diluent. Background-subtracted data values (mean +/- SD) are graphed.

  • Quantification of PAI interpolated from standard curve and multiplied by dilution factor.

プロトコール

参考文献

This product has been referenced in:

  • Kamtchum-Tatuene J  et al. HIV is associated with endothelial activation despite ART, in a sub-Saharan African setting. Neurol Neuroimmunol Neuroinflamm 6:e531 (2019). ELISA . Read more (PubMed: 30697583) »
  • Che Y  et al. Cisplatin-activated PAI-1 secretion in the cancer-associated fibroblasts with paracrine effects promoting esophageal squamous cell carcinoma progression and causing chemoresistance. Cell Death Dis 9:759 (2018). Read more (PubMed: 29988148) »
See all 5 Publications for this product

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