Human PAI1 + tPA ELISA Kit (ab108892)

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SimpleStep ELISA® なら結果まで 90 分

製品の概要

  • 製品名

    Human PAI1 + tPA ELISA Kit
    PAI1 + tPA キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Overall 5.2%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Overall 10.2%

  • サンプルの種類

    Cell culture supernatant, Serum, Plasma, Tissue

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    = 0.06 ng/ml

  • 検出範囲

    0.063 ng/ml - 4 ng/ml

  • 添加回収試験

    98 %

  • 全工程の試験時間

    4h 00m

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Abcam’s PAI1 + tPA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 + tPA concentrations in plasma, serum, tissue extracts and cell culture supernatants.


    A PAI1 + tPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 + tPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 + tPA captured in plate.


    Get results in 90 minutes with Human PAI1+tPA ELISA Kit (ab192151) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • 試験プラットフォーム

    Microplate

製品の特性

関連製品

画像

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108892.

参考文献

ab108892 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108892 は 2 報の論文で使用されています。

  • Zahran AM  et al. Circulating microparticle subpopulation in metabolic syndrome: relation to oxidative stress and coagulation markers. Diabetes Metab Syndr Obes 12:485-493 (2019). PubMed: 31043798
  • Valle-Garay E  et al. tPA Alu (I/D) polymorphism associates with bacterial osteomyelitis. J Infect Dis 208:218-23 (2013). Human . PubMed: 23570848

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Question

hello
I come from taiwan (asia).
I purchased the PAI-1/tPA Human ELISA kit (ab108892) to determine the PAI-
1/tPA complex.
But the results are not expected, so need more help from you.
Actually, my project is to compare the PAI-1 binding with free rtPA and
immobilized rtPA.
(immobilized rtAP: rtPA covalent bond with magnetic nanoparticles, it's MNP-
rtPA)
In vitro experiment,
rtPA (or MNP-rtPA) incubated with PAI-1 at different molar ratio followed by
ELISA kit was used.
The molar ratios of rtPA/PAI-1 are 0, 0.25, 0.5, 0.75 and 1.
As the [PAI-1] increased, i expected the PAI-1/tPA complex increased, too.
The attatched file is the results
The results shows that PAI-1/tPA complex was not increased with the molar
ratio of rtPA/PAI-1 incresed
I am also confused about the group of rtPA/PAI-1 (molar ratio: 0)
There is no PAI-1 in this group, why the OD 450 nm was the highest?
Please help me to resolve these quenstions or give me some commons, thanks a
lot.

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Answer

Thank you for taking the time to contact us. I am sorry to hear you have some concerns regarding the results from this kit.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forELISA on human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. I hope I will then be able to help answer your questions in more detail. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



A. General Information
- Catalogue number:

- Lot number:

- Purchase order number/ order date or preferably the Abcam order number:

- How was the kit stored?


B. Description of the problem
- Are the signals you obtained with control or standard satisfactory? (Yes/No);


- Are the sample readings as expected or inconsistent?


- Are the blank readings as expected?

- Other (e.g. missing components)


C. What do you think the problem is caused by? E.g. enzyme is faulty, developer is faulty or other component that you think might be faulty.



D. Sample
- Sample Type (e.g. Cell culture supernatant, Cell lysates, Tissue lysates or Serum samples)


- Sample Species (e.g. human, mouse or rat)


- Storage (at what temperature and for how long were the samples stored?)

- Sample treatment, if any



- Sometime proteins in samples interfere with readings. Have you removed proteins?


- Sample preparation


E. Protocol used:
Did you follow the protocol exactly as given on the datasheet?


F. Optimization attempts (problem solving); please describe any deviations from the protocol.



G. Have you used the same kit successfully before?


H. Do you obtain the same results every time?


I. Additional Notes; (any additional information you would like to provide)



J. Results:

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Question

Re Kits: ab108892- PAI-1 tPA Human ELISA kit ab46509- Thrombomodulin Human ELISA kit ab100656- Thrombopoietin Human ELISA kit I am looking for a panel of assays to detect thrombopoietin, PAI-1, and trhrombomodulin in cynomolgus monkey. I understand that the kits referenced above state their specificity to human, but can you tell me if they have been tested for cross reactivity to monkey?

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Answer

Thank you for contacting Abcam.
I have been in contact with the lab about the possibility of using ab108892 and ab46509 with samples from Cynomolgus Monkey. Unfortunately, the cross-reactivity is low so it will not work well for monkey samples.
If there is anything else I can help you with, please let me know.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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